anti-Cow (Bovine) CAMK2G antibody for Immunocytochemistry

Recommended CAMK2G Antibody (supplied by: Log in to see )

Antigen
Calcium/calmodulin-Dependent Protein Kinase II gamma (CAMK2G) Antibodies
  • CAMK
  • CAMK-II
  • CAMKG
  • 5930429P18Rik
  • Camkg
  • calcium/calmodulin dependent protein kinase II gamma
  • calcium/calmodulin-dependent protein kinase II gamma
  • CAMK2G
  • Camk2g
Reactivity
Cow (Bovine), Human, Mouse (Murine), Rat (Rattus)
103
74
72
21
3
3
3
2
2
2
1
1
1
1
Host
Mouse
66
48
Clonality
Monoclonal
Conjugate
This CAMK2G antibody is un-conjugated
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Radioimmunoassay (RIA), ELISA, Western Blotting (WB)
110
63
61
52
40
38
18
7
3
3
1
1
1
1
1
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

Submit your validation data for this product and get a full refund. I want to validate this product

Learn more

Pubmed
13 references available

Available images

image for anti-Calcium/calmodulin-Dependent Protein Kinase II gamma (CAMK2G) antibody (ABIN361644)
Western Blotting (WB) image for anti-Calcium/calmodulin-Dependent Protein Kinase II gamma (CAMK2G) antibody (ABIN361644)
Catalog No. ABIN361644
$ 349.80
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN2484551 ICC IF IP RIA IHC ELISA WB Atto 565 Mouse IgG1 Log in to see 6G9 2
1 ABIN2484560 ICC IF IP RIA IHC ELISA WB FITC Mouse IgG1 Log in to see 6G9 2
1 ABIN2484562 ICC IF IP RIA IHC ELISA WB PE-Atto 594 Mouse IgG1 Log in to see 6G9 2
1 ABIN2484552 ICC IF IP RIA IHC ELISA WB Atto 594 Mouse IgG1 Log in to see 6G9 2
1 ABIN2484559 ICC IF IP RIA IHC ELISA WB Biotin Mouse IgG1 Log in to see 6G9 2
1 ABIN2484565 ICC IF IP RIA IHC ELISA WB Streptavidin Mouse IgG1 Log in to see 6G9 2
1 ABIN2484558 ICC IF IP RIA IHC ELISA WB APC Mouse IgG1 Log in to see 6G9 2
1 ABIN2484554 ICC IF IP RIA IHC ELISA WB Atto 655 Mouse IgG1 Log in to see 6G9 2
1 ABIN2484549 ICC IF IP RIA IHC ELISA WB Atto 390 Mouse IgG1 Log in to see 6G9 2
1 ABIN2484561 ICC IF IP RIA IHC ELISA WB HRP Mouse IgG1 Log in to see 6G9 2
1 ABIN2484557 ICC IF IP RIA IHC ELISA WB Alkaline Phosphatase (AP) Mouse IgG1 Log in to see 6G9 2
1 ABIN2484563 ICC IF IP RIA IHC ELISA WB PerCP Mouse IgG1 Log in to see 6G9 2
1 ABIN2484564 ICC IF IP RIA IHC ELISA WB PE Mouse IgG1 Log in to see 6G9 2
1 ABIN2484550 ICC IF IP RIA IHC ELISA WB Atto 488 Mouse IgG1 Log in to see 6G9 2
1 ABIN2484555 ICC IF IP RIA IHC ELISA WB Atto 680 Mouse IgG1 Log in to see 6G9 2
1 ABIN2484553 ICC IF IP RIA IHC ELISA WB Atto 633 Mouse IgG1 Log in to see 6G9 2
1 ABIN2484556 ICC IF IP RIA IHC ELISA WB Atto 700 Mouse IgG1 Log in to see 6G9 2
1 ABIN1721474 ICC IF IHC IP RIA WB Mouse IgG1 Log in to see 6G9 0

General

Antigen Calcium/calmodulin-Dependent Protein Kinase II gamma (CAMK2G) Antibodies
Reactivity Cow (Bovine), Human, Mouse (Murine), Rat (Rattus)
(103), (74), (72), (21), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1)
Host Mouse
(66), (48)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CAMK2G antibody is un-conjugated
(3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Radioimmunoassay (RIA), ELISA, Western Blotting (WB)
(110), (63), (61), (52), (40), (38), (18), (7), (3), (3), (1), (1), (1), (1), (1)
Pubmed 13 references available
Supplier Log in to see

Product Details anti-CAMK2G Antibody

Target Details CAMK2G Application Details Handling References for anti-CAMK2G antibody (ABIN361644) Images
Specificity Detects ~50-60 kDa. Recognizes both phosphorylated and non-phosphorylated forms.
Purification Protein G Purified
Immunogen Partially purified rat CaMKII
Clone 6G9
Isotype IgG1

Target Details CAMK2G

Product Details anti-CAMK2G Antibody Application Details Handling References for anti-CAMK2G antibody (ABIN361644) Images back to top
Antigen
Alternative Name CaMKII (CAMK2G Antibody Abstract)
Background CaMKII is an important member of the calcium/calmodulin-activated protein kinase family, functioning in neural synaptic stimulation and T-cell receptor signaling (1, 2). CaMKII is expressed in many different tissues but is specifically found in the neurons of the forebrain and its mRNA is found within the dendrites and the soma of the neuron. The CaMKII that is found in the neurons consist of two subunits of 52 (termed alpha genes) and 60 kDa (beta genes). CaMKII has catalytic and regulatory domains, as well as an ATP-binding domain, and a consensus phosphorylation site (3-7). The binding of Ca2+/calmodulin to its regulatory domain releases its auto inhibitory effect and activates the kinase (8). This kinase activation results in autophosphorylation at threonine 286 (8). The threonine phosphorylation state of CaMKII can be regulated through PP1/PKA. Whereas PP1 (protein phosphatase 1) dephosphorylates phospho-CaMKII at Thr286, PKA (protein kinase A) prevents this dephosphorylation (9). Autophosphorylation also enables CaMKII to attain an enhanced affinity for NMDA receptors in postsynaptic densities (10-12).
Gene ID 12322
NCBI Accession NP_033922
UniProt P11798
Pathways WNT Signaling, Interferon-gamma Pathway, Hormone Transport, Myometrial Relaxation and Contraction, Regulation of long-term Neuronal Synaptic Plasticity

Application Details

Product Details anti-CAMK2G Antibody Target Details CAMK2G Handling References for anti-CAMK2G antibody (ABIN361644) Images back to top
Application Notes
  • WB (1:10000)
  • IHC (1:2000)
  • ICC/IF (1:50)
  • optimal dilutions for assays should be determined by the user.
Comment

0.1 μg/ml was sufficient for detection of CamKII in 20 μg rat brain tissue extract by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:AP as the secondary.
Restrictions For Research Use only

Handling

Product Details anti-CAMK2G Antibody Target Details CAMK2G Application Details References for anti-CAMK2G antibody (ABIN361644) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C

References for anti-CAMK2G antibody (ABIN361644)

Product Details anti-CAMK2G Antibody Target Details CAMK2G Application Details Handling Images back to top
Product cited in:

Feng, Szabo, Dziak, Opas: "Cytoskeletal disassembly and cell rounding promotes adipogenesis from ES cells." in: Stem cell reviews, Vol. 6, Issue 1, pp. 74-85, 2010

Background publications

Hughes, Edin, Antonsson, Grundström: "Calmodulin-dependent kinase II mediates T cell receptor/CD3- and phorbol ester-induced activation of IkappaB kinase." in: The Journal of biological chemistry, Vol. 276, Issue 38, pp. 36008-13, 2001

Means: "Regulatory cascades involving calmodulin-dependent protein kinases." in: Molecular endocrinology (Baltimore, Md.), Vol. 14, Issue 1, pp. 4-13, 2000

Leonard, Lim, Hemsworth, Horne, Hell: "Calcium/calmodulin-dependent protein kinase II is associated with the N-methyl-D-aspartate receptor." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, Issue 6, pp. 3239-44, 1999

Makhinson, Chotiner, Watson, ODell: "Adenylyl cyclase activation modulates activity-dependent changes in synaptic strength and Ca2+/calmodulin-dependent kinase II autophosphorylation." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 19, Issue 7, pp. 2500-10, 1999

Shen, Meyer: "Dynamic control of CaMKII translocation and localization in hippocampal neurons by NMDA receptor stimulation." in: Science (New York, N.Y.), Vol. 284, Issue 5411, pp. 162-6, 1999

Strack, Colbran: "Autophosphorylation-dependent targeting of calcium/ calmodulin-dependent protein kinase II by the NR2B subunit of the N-methyl- D-aspartate receptor." in: The Journal of biological chemistry, Vol. 273, Issue 33, pp. 20689-92, 1998

Barria, Muller, Derkach, Griffith, Soderling: "Regulatory phosphorylation of AMPA-type glutamate receptors by CaM-KII during long-term potentiation." in: Science (New York, N.Y.), Vol. 276, Issue 5321, pp. 2042-5, 1997

Tombes, Krystal: "Identification of novel human tumor cell-specific CaMK-II variants." in: Biochimica et biophysica acta, Vol. 1355, Issue 3, pp. 281-92, 1997

Brocke, Srinivasan, Schulman: "Developmental and regional expression of multifunctional Ca2+/calmodulin-dependent protein kinase isoforms in rat brain." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 15, Issue 10, pp. 6797-808, 1995

Edman, Schulman: "Identification and characterization of delta B-CaM kinase and delta C-CaM kinase from rat heart, two new multifunctional Ca2+/calmodulin-dependent protein kinase isoforms." in: Biochimica et biophysica acta, Vol. 1221, Issue 1, pp. 89-101, 1994

Nghiem, Saati, Martens, Gardner, Schulman: "Cloning and analysis of two new isoforms of multifunctional Ca2+/calmodulin-dependent protein kinase. Expression in multiple human tissues." in: The Journal of biological chemistry, Vol. 268, Issue 8, pp. 5471-9, 1993

Bennett, Kennedy: "Deduced primary structure of the beta subunit of brain type II Ca2+/calmodulin-dependent protein kinase determined by molecular cloning." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 84, Issue 7, pp. 1794-8, 1987

Images

Product Details anti-CAMK2G Antibody Target Details CAMK2G Application Details Handling References for anti-CAMK2G antibody (ABIN361644) back to top
Supplier Images
image for anti-Calcium/calmodulin-Dependent Protein Kinase II gamma (CAMK2G) antibody (ABIN361644) CamKII (6G9) in cultured cortical neurons.
Western Blotting (WB) image for anti-Calcium/calmodulin-Dependent Protein Kinase II gamma (CAMK2G) antibody (ABIN361644) western rat membrane 1 in 1000 CaMKII.