anti-Human FZD10 antibody for Immunohistochemistry

Recommended FZD10 Antibody (supplied by: Log in to see )

Antigen
Frizzled Family Receptor 10 (FZD10) Antibodies
  • CD350
  • FZ-10
  • Fz10
  • FzE7
  • hFz10
  • cFz-10
  • Fz-10
  • Xfr9
  • Xfz10
  • frizzled-10
  • frizzled10
  • fz10
  • fze7
  • hfz10
  • fk48e04
  • fz4
  • fzb
  • wu:fk48e04
  • zg04
  • Fzd10
  • Xfz10B
  • Xfz9
  • fzd10b
  • fzd9
  • frizzled class receptor 10
  • frizzled class receptor 2
  • frizzled class receptor 10 S homeolog
  • FZD10
  • fzd10
  • Fzd10
  • Fzd2
  • fzd10.S
Epitope
N-Term
15
10
5
4
4
3
2
1
1
1
1
1
Reactivity
Human, Monkey
65
53
25
7
4
4
4
3
2
2
1
Host
Rabbit
72
Clonality
Polyclonal
Conjugate
This FZD10 antibody is un-conjugated
3
3
2
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
44
27
16
14
13
9
3
3
1
1
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Catalog No. ABIN1048619
$ 484.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
9.312915 ABIN2432401 IHC ELISA Rabbit IgG Log in to see Polyclonal 0
1 ABIN3184680 ELISA IF IHC WB Rabbit IgG Internal Region Log in to see Polyclonal 0
1 ABIN4312584 ICC IF IHC IHC (p) Rabbit IgG Log in to see Polyclonal 0
1 ABIN2892419 IF IHC WB Rabbit Leu164 Log in to see Polyclonal 0
1 ABIN4312583 ICC IF IHC IHC (p) Rabbit IgG Log in to see Polyclonal 0
1 ABIN2270494 IHC Rabbit N-Term Log in to see Polyclonal 0
1 ABIN6102418 ELISA IF/ICC IHC WB Rabbit IgG Internal Region Log in to see Polyclonal 0
1 ABIN6115514 ELISA IF/ICC IHC WB Rabbit IgG AA 125-225 Log in to see Polyclonal 0
1 ABIN6040559 ELISA IHC Rabbit IgG Log in to see Polyclonal 0
1 ABIN5578500 IF IHC WB Rabbit Log in to see Polyclonal 0
1 ABIN5702679 ELISA IHC WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN5917871 ELISA IHC WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN5996607 IHC WB Rabbit Log in to see Polyclonal 0

General

Antigen Frizzled Family Receptor 10 (FZD10) Antibodies
Epitope N-Term
(15), (10), (5), (4), (4), (3), (2), (1), (1), (1), (1), (1)
Reactivity Human, Monkey
(65), (53), (25), (7), (4), (4), (4), (3), (2), (2), (1)
Host Rabbit
(72)
Clonality
Polyclonal
Conjugate This FZD10 antibody is un-conjugated
(3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(44), (27), (16), (14), (13), (9), (3), (3), (1), (1)
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Product Details anti-FZD10 Antibody

Target Details FZD10 Application Details Handling Images
Brand IHC-plus™
Specificity Human FZD10 / Frizzled 10. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Predicted Reactivity Percent identity with other species by BLAST analysis: Human, Monkey (100%) Gibbon, Hamster, Panda, Dog (93%) Marmoset, Mouse, Bovine, Horse, Pig (86%).
Purification Immunoaffinity purified
Immunogen Synthetic 14 amino acid peptide from N-terminal extracellular domain of human FZD10 / Frizzled 10. Percent identity with other species by BLAST analysis: Human, Monkey (100%), Gibbon, Hamster, Panda, Dog (93%), Marmoset, Mouse, Bovine, Horse, Pig (86%).

Type of Immunogen: Synthetic peptide
Plasmids, Primers & others

Target Details FZD10

Product Details anti-FZD10 Antibody Application Details Handling Images back to top
Antigen
Alternative Name FZD10 / Frizzled 10 (FZD10 Antibody Abstract)
Background Name/Gene ID: FZD10
Subfamily: Frizzled
Family: GPCR

Synonyms: FZD10, CD350 antigen, CD350, FZ-10, Frizzled homolog 10, HFz10, Frizzled 10, Frizzled family receptor 10, Frizzled-10, FzE7, Fz10
Gene ID 11211
Pathways WNT Signaling

Application Details

Product Details anti-FZD10 Antibody Target Details FZD10 Handling Images back to top
Application Notes Approved: IHC, IHC-P (6 - 16 μg/mL)
Comment

Target Species of Antibody: Human

Assay Procedure The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip
Restrictions For Research Use only

Handling

Product Details anti-FZD10 Antibody Target Details FZD10 Application Details Images back to top
Format Liquid
Concentration Lot specific
Buffer PBS, less than 0.1 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Expiry Date 12 months

Images

Product Details anti-FZD10 Antibody Target Details FZD10 Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-Frizzled Family Receptor 10 (FZD10) (N-Term) antibody (ABIN1048619) Anti-FZD10 / Frizzled 10 antibody ABIN1048619 IHC staining of human kidney, fetal. I...