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The centromere is a specialized chromatin domain, present throughout the cell cycle, that acts as a platform on which the transient assembly of the kinetochore occurs during mitosis. Additionally we are shipping CENPT Kits (15) and CENPT Proteins (5) and many more products for this protein.
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These results support the existence of 2 pathways for kinetochore assembly directed by CENP-C (show CENPC1 Antibodies) and CENP-T/W, which can be reconstituted in Xenopus egg extracts.
Whereas CENP-C (show CENPC1 Antibodies) recruits a single MIS12 (show MIS12 Antibodies):NDC80 (show NDC80 Antibodies) complex, the authors show here that CENP-T binds one MIS12 (show MIS12 Antibodies):NDC80 (show NDC80 Antibodies) and two NDC80 (show NDC80 Antibodies) complexes upon phosphorylation by the mitotic CDK1 (show CDK1 Antibodies):Cyclin B complex at three distinct CENP-T sites.
FACT chaperone stabilizes the soluble CENP-T/-W complex in the cell and promotes dynamics of exchange, enabling CENP-T/-W deposition at centromeres.
We used a synthetic system to dissect how CenH3(CENP-A (show CENPA Antibodies)) contributes to the accumulation of CENP-C (show CENPC1 Antibodies) and CENP-T, two key components that are necessary for the formation of functional kinetochores
CENP-T directly interacts with Ndc80 (show NDC80 Antibodies), which in turn promotes KNL1 (show CASC5 Antibodies)/Mis12 (show MIS12 Antibodies) complex recruitment at kinetochores during chromosome segregation.
CSN5 (show COPS5 Antibodies) regulates the stability of the inner kinetochore components CENP-T and CENP-W (show CENPW Antibodies), providing the first direct link between CSN5 (show COPS5 Antibodies) and the mitotic apparatus, highlighting the role of CSN5 (show COPS5 Antibodies) as a multifunctional cell cycle regulator (show CDKN2A Antibodies).
CENP-T provides a structural platform for outer kinetochore assembly.
results reveal the molecular function of CENP-T proteins and demonstrate how the Ndc80 (show NDC80 Antibodies) complex is anchored to centromeres in a manner that couples chromosome movement to spindle dynamics
The CENP-T/W complex assembles through a dynamic exchange mechanism in late S-phase and G2, is required for mitosis in each cell cycle and does not persist across cell generations.
Study demonstrates that CENP-W (show CENPW Antibodies) forms a DNA-binding complex together with the CCAN component CENP-T; this complex directly associates with nucleosomal DNA and with canonical histone H3 (show HIST3H3 Antibodies), but not with CENP-A (show CENPA Antibodies), in centromeric regions.
CENP-T exchange into centromeres is restricted to the S-phase of the cell cycle as revealed by FRAP (show FRAP1 Antibodies), suggesting a coreplicational loading mechanism.
The centromere is a specialized chromatin domain, present throughout the cell cycle, that acts as a platform on which the transient assembly of the kinetochore occurs during mitosis. All active centromeres are characterized by the presence of long arrays of nucleosomes in which CENPA (MIM 117139) replaces histone H3 (see MIM 601128). CENPT is an additional factor required for centromere assembly (Foltz et al., 2006
centromere protein T
, centromere protein T-like
, interphase centromere complex protein 22