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Indoleamine 2,3-Dioxygenase 1 (IDO1) antibody

Details for Product No. ABIN1043734
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Antigen
Synonyms IDO, IDO-1, INDO, Ido, Indo, ido, indo, cd107b, IDO1
Reactivity
Mouse (Murine), Human
(79), (33), (19), (18), (12), (12)
Host
Mouse
(50), (33), (7), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunoprecipitation (IP)
(73), (45), (20), (16), (10), (9), (7), (4), (4), (3), (3)
Pubmed 1 reference available
Quantity 25 µL
Options
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Catalog No. ABIN1043734
126.50 $
Plus shipping costs $45.00 and if applicable $26.40 dry ice

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen Anti-IDO1 (MOUSE) Monoclonal Antibody was produced in mouse by repeated immunizations with fragment of recombinant human and mouse IDO1 protein followed by hybridoma development.
Immunogen Type: RecombinantProtein
Clone 10.1
Isotype IgG3
Specificity Anti-IDO1 was purified from concentrated tissue culture supernate by Protein G chromatography followed by extensive dialysis against the buffer stated above. This antibody is specific for human and mouse IDO1 protein. A BLAST analysis was used to suggest cross-reactivity with IDO1 from human and mouse sources based on 100% homology with the immunizing sequence. Cross-reactivity with IDO1 from other sources has not been determined.
Characteristics Indoleamine 2, 3-dioxygenase1 (IDO1) is a 41-42 kD intracellular enzyme that catabolizes tryptophan into kynurenine. IDO1 modulates levels of the amino acid tryptophan, which is vital for cell growth, but is also involved in the suppression of the immune response. IDO1 effects on immune suppression are due to decreased tryptophan availability and the generation of tryptophan metabolites, resulting in negative effects on T lymphocytes, including proliferation, function and survival. IDO1 may be involved in the suppression of the immune response to tumors, and blocking the IDO1 pathway may be a potential target for immuno and cancer therapy. IDO1 is expressed in a wide variety of tissues and can be upregulated by interferon gamma and other inflammatory cytokines.
Sterility Sterile filtered
Alternative Name IDO1
Background Indoleamine 2, 3-dioxygenase1 (IDO1) is a 41-42 kD intracellular enzyme that catabolizes tryptophan into kynurenine. IDO1 modulates levels of the amino acid tryptophan, which is vital for cell growth, but is also involved in the suppression of the immune response. IDO1 effects on immune suppression are due to decreased tryptophan availability and the generation of tryptophan metabolites, resulting in negative effects on T lymphocytes, including proliferation, function and survival. IDO1 may be involved in the suppression of the immune response to tumors, and blocking the IDO1 pathway may be a potential target for immuno and cancer therapy. IDO1 is expressed in a wide variety of tissues and can be upregulated by interferon gamma and other inflammatory cytokines.
Synonyms: Ido, Indo, Indoleamine 2,3-dioxygenase 1, Indoleamine-pyrrole 2,3-dioxygenase, Ido1, Ido-1
Gene ID 3620
NCBI Accession NP_002155.1
UniProt P14902
Application Notes Anti-IDO1 antibody has been tested for use in ELISA, Western Blot, IP, IHC, and Flow Cytometry. Specific conditions for reactivity should be optimized by the end user.
Comment

Gene Name: Ido1

Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  Expiration date is one (1) year from date of opening.
Expiry Date 12 months
Supplier Images
anti-Indoleamine 2,3-Dioxygenase 1 (IDO1) antibody Western Blot of Mouse Anti-IDO1 Antibody. Lane 1: untreated HeLa cells. Lane 2: IFN-r treated HeLa cells. Load: 35 µg per lane. Primary antibody: IDO 1 Antibody at 1:1000 for overnight at 4°C. Secondary antibody: IRDye800™ mouse secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 41-42 kDa, 41-42 kDa for IDO-1. Other band(s): none.
Product cited in: Mellor, Munn: "IDO expression by dendritic cells: tolerance and tryptophan catabolism." in: Nature reviews. Immunology, Vol. 4, Issue 10, pp. 762-74, 2004 (PubMed).

Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN1043734
126.50 $
Plus shipping costs $45.00 and if applicable $26.40 dry ice

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Validation Images
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