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Tumor Necrosis Factor antibody (TNF)

Details for Product anti-TNF Antibody No. ABIN1043796, Supplier: Log in to see
Antigen
  • TNF
  • tnf
  • dif
  • tnfa
  • xtnf
  • TNF-a
  • tnfsf2
  • tnf-alpha
  • TNF-alpha
  • DIF
  • TNFA
  • TNFSF2
  • TNFalpha
  • Tnfa
  • Tnfsf1a
  • RATTNF
  • TNFa
  • cTNF
  • TNF-ALPHA
  • Cachectin
  • Tnf-alpha
  • tnfa-like
Alternatives
anti-Human Tumor Necrosis Factor antibody for ELISA
Reactivity
Human
706
308
142
55
53
32
31
27
23
18
12
12
8
7
5
4
4
4
3
3
2
2
1
1
1
Host
Rabbit
538
395
157
43
13
9
9
7
4
1
Clonality
Polyclonal
Conjugate
This Tumor Necrosis Factor antibody is un-conjugated
147
83
44
24
20
9
9
7
5
4
4
4
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
Application
Neutralization (Neut), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
632
586
319
207
119
100
99
86
80
63
56
56
25
25
24
22
15
14
8
8
6
6
5
5
4
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
Supplier
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Immunogen The whole rabbit serum used to produce this IgG fraction antibody was prepared by repeated immunizations with recombinant human TNFalpha produced in E.coli.
Immunogen Type: RecombinantProtein
Isotype IgG
Specificity This antibody is primarily directed against mature 17,000 MW human TNFalpha and is useful in determining its presence in various assays. In general, this antibody also detects primate TNFalpha in the same formats using similar dilutions. The antibody does not recognize human TNGß (lymphotoxin). This IgG fraction antibody will recognize the cell-bound precursor of TNFalpha as a 26,000 protein in immunoblots, particularly in denatured samples. This antibody is also useful for neutralization of human and primate TNFalpha activity in bioassays. It does not neutralize the biological activity of lymphotoxin. For neutralization, it is recommended to incubate the sample with a 1:200 dilution of the antibody for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG is recommended.
Characteristics Anti TNF alpha Antibody recognizes TNF alpha (TNF, cachexin, cachectin, tumor necrosis factor-alpha or TNF-alpha) a cytokine involved in systemic inflammation. TNF alpha is a member of a group of cytokines that stimulate the acute phase reaction. It is produced chiefly by activated macrophages, although it can be produced by other cell types as well. The primary role of TNF alpha is in the regulation of immune cells. TNFis an endogenous pyrogen that is able to induce fever, apoptotic cell death, sepsis (through IL-1 & IL-6 production), cachexia, inflammation, and to inhibit tumorigenesis and viral replication. Dysregulation of TNF production has been implicated in a variety of human diseases, including Alzheimer's disease, cancer, major depression, and inflammatory bowel disease (IBD).
Sterility Sterile filtered
Alternative Name TNF alpha (TNF Antibody Abstract)
Background Anti TNF alpha Antibody recognizes TNF alpha (TNF, cachexin, cachectin, tumor necrosis factor-alpha or TNF-α) a cytokine involved in systemic inflammation. TNF alpha is a member of a group of cytokines that stimulate the acute phase reaction. It is produced chiefly by activated macrophages, although it can be produced by other cell types as well. The primary role of TNF alpha is in the regulation of immune cells. TNFis an endogenous pyrogen that is able to induce fever, apoptotic cell death, sepsis (through IL-1 & IL-6 production), cachexia, inflammation, and to inhibit tumorigenesis and viral replication. Dysregulation of TNF production has been implicated in a variety of human diseases, including Alzheimer's disease, cancer, major depression, and inflammatory bowel disease (IBD).
Synonyms: APC1 antibody, Cachectin antibody, DIF antibody, Differentiation inducing factor antibody, Macrophage cytotoxic factor antibody, MCF antibody, Necrosin antibody
Gene ID 7124
UniProt P01375
Research Area Cardiovascular, Atherosclerosis, Growth Factors, Innate Immunity, Cytokines, Receptors, Immunology, Inflammation, Cancer, Virology
Pathways NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis
Application Notes This IgG fraction antibody of anti-Human TNFα has been tested for use in neutralizations, ELISA, immunohistochemistry and immunoblotting. It recognizes the 17,000 MW TNFα. Reactivity in other immunoassays is unknown.
Comment

Gene Name: TNFA

Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative Without preservative
Storage 4 °C/-20 °C
Storage Comment Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.
Expiry Date 12 months
Supplier Images
Immunohistochemistry (IHC) image for anti-Tumor Necrosis Factor antibody (TNF) (ABIN1043796) Immunohistochemistry using polyclonal TNFa antibody showing staining of formalin/PFA-...
 image for anti-Tumor Necrosis Factor antibody (TNF) (ABIN1043796) anti-Tumor Necrosis Factor (TNF) antibody (Image 2)
 image for anti-Tumor Necrosis Factor antibody (TNF) (ABIN1043796) anti-Tumor Necrosis Factor (TNF) antibody (Image 3)
Product cited in: Kant, Swat, Zhang et al.: "TNF-stimulated MAP kinase activation mediated by a Rho family GTPase signaling pathway." in: Genes & development, Vol. 25, Issue 19, pp. 2069-78, 2011 (PubMed).

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