Tumor Necrosis Factor antibody (TNF)

Details for Product anti-TNF Antibody No. ABIN1043796, Supplier: Log in to see
Antigen
  • TNF
  • tnf
  • dif
  • tnfa
  • xtnf
  • TNF-a
  • tnfsf2
  • tnf-alpha
  • TNF-alpha
  • DIF
  • TNFA
  • TNFSF2
  • TNFalpha
  • Tnfa
  • Tnfsf1a
  • RATTNF
  • TNFa
  • cTNF
  • TNF-ALPHA
  • Cachectin
  • Tnf-alpha
  • tnfa-like
  • tumor necrosis factor (TNF superfamily, member 2)
  • tumor necrosis factor
  • tumour necrosis factor alpha-like
  • tumor necrosis factor alpha
  • lipopolysaccharide-induced TNF factor
  • tumor necrosis factor b (TNF superfamily, member 2)
  • TNF
  • tnf
  • tnf-alpha
  • LITAF
  • Tnf
  • tnfb
Alternatives
anti-Human Tumor Necrosis Factor antibody for Activity Inhibition
Reactivity
Human
1254
460
358
145
136
119
119
63
55
41
29
18
14
12
9
7
6
4
4
4
4
2
1
1
1
1
Host
Rabbit
940
515
184
48
10
9
7
6
4
3
2
Clonality
Polyclonal
Conjugate
This Tumor Necrosis Factor antibody is un-conjugated
169
100
74
50
32
30
28
27
26
25
25
25
25
25
24
24
24
23
13
9
4
4
4
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
Application
Neutralization (Neut), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
886
858
622
322
285
230
219
190
136
118
98
74
29
27
26
22
20
18
14
9
7
7
6
6
6
5
5
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Immunogen The whole rabbit serum used to produce this IgG fraction antibody was prepared by repeated immunizations with recombinant human TNFalpha produced in E.coli.
Immunogen Type: RecombinantProtein
Isotype IgG
Specificity This antibody is primarily directed against mature 17,000 MW human TNFalpha and is useful in determining its presence in various assays. In general, this antibody also detects primate TNFalpha in the same formats using similar dilutions. The antibody does not recognize human TNGß (lymphotoxin). This IgG fraction antibody will recognize the cell-bound precursor of TNFalpha as a 26,000 protein in immunoblots, particularly in denatured samples. This antibody is also useful for neutralization of human and primate TNFalpha activity in bioassays. It does not neutralize the biological activity of lymphotoxin. For neutralization, it is recommended to incubate the sample with a 1:200 dilution of the antibody for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG is recommended.
Characteristics Anti TNF alpha Antibody recognizes TNF alpha (TNF, cachexin, cachectin, tumor necrosis factor-alpha or TNF-alpha) a cytokine involved in systemic inflammation. TNF alpha is a member of a group of cytokines that stimulate the acute phase reaction. It is produced chiefly by activated macrophages, although it can be produced by other cell types as well. The primary role of TNF alpha is in the regulation of immune cells. TNFis an endogenous pyrogen that is able to induce fever, apoptotic cell death, sepsis (through IL-1 & IL-6 production), cachexia, inflammation, and to inhibit tumorigenesis and viral replication. Dysregulation of TNF production has been implicated in a variety of human diseases, including Alzheimer's disease, cancer, major depression, and inflammatory bowel disease (IBD).
Sterility Sterile filtered
Alternative Name TNF alpha (TNF Antibody Abstract)
Background Anti TNF alpha Antibody recognizes TNF alpha (TNF, cachexin, cachectin, tumor necrosis factor-alpha or TNF-α) a cytokine involved in systemic inflammation. TNF alpha is a member of a group of cytokines that stimulate the acute phase reaction. It is produced chiefly by activated macrophages, although it can be produced by other cell types as well. The primary role of TNF alpha is in the regulation of immune cells. TNFis an endogenous pyrogen that is able to induce fever, apoptotic cell death, sepsis (through IL-1 & IL-6 production), cachexia, inflammation, and to inhibit tumorigenesis and viral replication. Dysregulation of TNF production has been implicated in a variety of human diseases, including Alzheimer's disease, cancer, major depression, and inflammatory bowel disease (IBD).
Synonyms: APC1 antibody, Cachectin antibody, DIF antibody, Differentiation inducing factor antibody, Macrophage cytotoxic factor antibody, MCF antibody, Necrosin antibody
Gene ID 7124
UniProt P01375
Research Area Cardiovascular, Atherosclerosis, Growth Factors, Innate Immunity, Cytokines, Receptors, Immunology, Inflammation, Cancer, Virology
Pathways NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus
Application Notes This IgG fraction antibody of anti-Human TNFα has been tested for use in neutralizations, ELISA, immunohistochemistry and immunoblotting. It recognizes the 17,000 MW TNFα. Reactivity in other immunoassays is unknown.
Comment

Gene Name: TNFA

Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative Without preservative
Storage 4 °C/-20 °C
Storage Comment Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.
Expiry Date 12 months
Supplier Images
Immunohistochemistry (IHC) image for anti-Tumor Necrosis Factor antibody (TNF) (ABIN1043796) Immunohistochemistry using polyclonal TNFa antibody showing staining of formalin/PFA-...
 image for anti-Tumor Necrosis Factor antibody (TNF) (ABIN1043796) anti-Tumor Necrosis Factor (TNF) antibody (Image 2)
 image for anti-Tumor Necrosis Factor antibody (TNF) (ABIN1043796) anti-Tumor Necrosis Factor (TNF) antibody (Image 3)
Product cited in: Kant, Swat, Zhang, Zhang, Neel, Flavell, Davis: "TNF-stimulated MAP kinase activation mediated by a Rho family GTPase signaling pathway." in: Genes & development, Vol. 25, Issue 19, pp. 2069-78, 2011 (PubMed).

Did you look for something else?