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PCNA antibody
PCNA
Reactivity: Human, Mouse, Rat
WB, IF, IHC (p), IHC (fro)
Host: Mouse
Monoclonal
IML-83
unconjugated
Product Details anti-PCNA Antibody
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Target
See all PCNA Antibodies
PCNA
(Proliferating Cell Nuclear Antigen (PCNA))
Reactivity
All reactivities for PCNA antibodies
Human, Mouse, Rat
Host
All hosts for PCNA antibodies
Mouse
Clonality
All clonalities for PCNA antibodies
Monoclonal
Conjugate
All conjugates for PCNA antibodies
This PCNA antibody is un-conjugated
Application
All applications for PCNA antibodies
Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
Specificity
This antibody reacts to PCNA.
Purification
Affinity chromatography
Immunogen
Protein A fusion protein
Clone
IML-83
Isotype
IgG2a
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Alternatives
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Application Details
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Application Notes
Optimal working dilution should be determined by the investigator.
Restrictions
For Research Use only
Handling
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Reconstitution
Restore with 1.2 % sodium acetate or neutral PBS
Concentration
0,1 mg/mL
Buffer
1.2 % sodium acetate, with 2 mg BSA and 0.01 mg sodium azide as preservative.
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing.
Storage
-20 °C
Storage Comment
Prior to reconstitution store at -20 °C. Following reconstitution store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Target Details for PCNA
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Target
PCNA
(Proliferating Cell Nuclear Antigen (PCNA))
Alternative Name
PCNA (PCNA Products )
Synonyms
PCNAR antibody, Pcna/cyclin antibody, pcna antibody, AO090003000846 antibody, 53/13 antibody, CG9193 antibody, DmPCNA antibody, Dmel\\CG9193 antibody, MUS209 antibody, Mus209 antibody, PCNA antibody, PCNA/mus209 antibody, Pcna antibody, dPCNA antibody, l(2)02448 antibody, l(2)56F antibody, l(2)56Fa antibody, l(2)s1534 antibody, cb16 antibody, fb36g03 antibody, wu:fa28e03 antibody, wu:fb36g03 antibody, pcna-A antibody, proliferating cell nuclear antigen antibody, DNA polymerase III sliding clamp antibody, Proliferating cell nuclear antigen antibody, Proliferating cell nuclear antigen-like antibody, proliferating cell nuclear antigen L homeolog antibody, proliferating cell nuclear antigen 1 antibody, PCNA antibody, Pcna antibody, pcna antibody, MMP_RS08810 antibody, ANI_1_512014 antibody, AOR_1_1500154 antibody, MGC53867 antibody, pcna.L antibody, pcna1 antibody, LOC106420145 antibody, pcn1 antibody
Background
Proliferating cell nuclear antigen (PCNA) was originally identified by immunofluorescence as a nuclear protein whose appearance correlated with the proliferative state of the cell. PCNA /cyclin has been localized by in situ hybridization to the short arm of human chromosome 20 with a peak of grains over band 20p13. PCNA gene is present in single copy and has 6 exons. It spans 4,961 bp. Synthesis of the nuclear protein cyclin and DNA in quiescent mouse fibroblasts is coordinately induced by serum and purified growth factors. PCNA controls establishment of sister chromatid cohesion during S phase.Synonyms: Cyclin, Proliferating Cell Nuclear Antigen
Gene ID
5111
NCBI Accession
NP_002583
UniProt
P12004
Pathways
Telomere Maintenance , DNA Damage Repair , Mitotic G1-G1/S Phases , DNA Replication , Synthesis of DNA , Autophagy
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