ERCC1 antibody

Catalog No. ABIN115126

Product Details anti-ERCC1 Antibody

Target: ERCC1 (Excision Repair Cross Complementing Polypeptide-1 (ERCC1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ERCC1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Specificity: This antibody reacts with a 33-36 kDa protein known as ERCC1 polypeptide. Cellular localization: Nuclear. Cross reacts with Rat.

Purification: Protein A Chromatography.

Immunogen: BALB/C mice were injected with full length recombinant human ERCC1 protein.

Clone: 8F1

Isotype: IgG2b

Application Details

Application Notes: Immunoprecipitation. Immunohistochemistry on Paraffin Embedded Sections: 1/100-1/200 in an ABC method. Formalin fixed paraffin embedded tissue sections require high temperature antigenunmasking with 10 mM citrate buffer, pH 6.0 prior to Immunostaining. Incubation Time: 30 minutes at RT. Recommended Positive Control: Tonsil. Western Blot: 1/100-1/500. use Human lung cell line as Positive Control.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

Target Details for ERCC1

Target: ERCC1 (Excision Repair Cross Complementing Polypeptide-1 (ERCC1))

Alternative Name: ERCC1 (ERCC1 Products)

Synonyms: COFS4 antibody, RAD10 antibody, UV20 antibody, Ercc-1 antibody, ercc1 antibody, T12H1.18 antibody, T12H1_18 antibody, UV REPAIR DEFICIENT 7 antibody, UVR7 antibody, ERCC excision repair 1, endonuclease non-catalytic subunit antibody, excision repair cross-complementing rodent repair deficiency, complementation group 1 antibody, DNA excision repair protein ERCC-1 antibody, nucleotide repair protein antibody, excision repair cross-complementation group 1 L homeolog antibody, excision repair cross-complementation group 1 antibody, ERCC1 antibody, Ercc1 antibody, CC1G_04546 antibody, CpipJ_CPIJ013903 antibody, PITG_13719 antibody, ercc1 antibody, ercc1.L antibody

Background: The mammalian ERCC1 (Excision Repair Cross Complementing) polypeptide is required for nucleotide excision repair (NER) of damaged DNA and is homologous to Saccharomyces cerevisiae RAD10, which functions in repair and mitotic intrachromosomal recombination. NER mechanism involves dual incisions on both sides of the damage catalyzed by two nucleases. In mammalian cells XPG cleaves 3' of the DNA lesion while the ERCC1-XPF complex makes the 5' incision. Elevated levels of ERCC1 have also been reported in Cisplatin-resistant cells.Synonyms: COFS4, DNA excision repair protein ERCC-1, UV20

Gene ID: 2067

NCBI Accession: NP_001974

UniProt: P07992

Pathways: DNA Damage Repair, Production of Molecular Mediator of Immune Response