GFP antibody (Green Fluorescent Protein) (TRITC)

Details for Product anti-GFP Antibody No. ABIN116750, Supplier: Log in to see
Antigen
  • green fluorescent protein
  • gfp
Reactivity
Aequorea victoria
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Host
Goat
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Clonality
Polyclonal
Conjugate
This GFP antibody is conjugated to TRITC
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Application
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Western Blotting (WB)
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Immunogen GST-Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246 aa) derived from the jellyfish Aequorea victoria
Specificity This antibody detects Fluorescent Protein (GFP). Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, anti-Peroxidase and purified and partially purified Green Fluorescent Protein (Aequorea victoria) Serum. No reaction was observed against Human, Mouse and Rat Serum Proteins.
Characteristics Absorption / Emission: 550 nm / 570 nm
Molar Ratio: 2.9 moles TRITC per mole of Goat IgG
Purification Immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities
Alternative Name GFP
Target Type Tag
Background Green fluorescence protein (GFP) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelenth of 509 nm) when excited by blue light (excitation peak at a wavelenth of 395 nm). Green Fluorescent Protein (GFP) has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. GFP technnology has considerably contributed to a greater understanding of cellular physiology. YFP differs from GFP due to a mutation at T203Y, antibodies raised against full-length GFP should also detect YFP and other variants.Synonyms: GFP-Tag, Green fluorescent protein
Gene ID 6100
UniProt P42212
Research Area Tags/Labels
Application Notes ELISA (Sandwich or Capture, ELISA for direct binding recognizes wild type, recombinantand enhanced forms of GFP, for sandwich ELISA titrate GFP in solution using either form ofthe antibody as the capture or detection antibodies, detection antibody is typically
Restrictions For Research Use only
Reconstitution Restore with 1.0 mL of deionized water (or equivalent).
Concentration 1.0 mg/mL (by UV absorbance at 280 nm)
Buffer 0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0 as a buffer, 10 mg/mL Bovine Serum Albumin (BSA) IgG and (protease free) as stabilizer, 0.01 % (w/v) Sodium azide as preservative
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing. Protect from light.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Product cited in: Niv, Keiner, Krishna, Witte, Lie, Redecker: "Aberrant neurogenesis after stroke: a retroviral cell labeling study." in: Stroke; a journal of cerebral circulation, Vol. 43, Issue 9, pp. 2468-75, 2012 (PubMed).

Suzuki, Mogami, Ihara, Urano: "Unique secretory dynamics of tissue plasminogen activator and its modulation by plasminogen activator inhibitor-1 in vascular endothelial cells." in: Blood, Vol. 113, Issue 2, pp. 470-8, 2009 (PubMed).

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