You are viewing an incomplete version of our website. Please click to reload the website as full version.

CD4 antibody (CD4 Molecule)

Details for Product anti-CD4 Antibody No. ABIN118486, Supplier: Log in to see
Antigen
  • cd4
  • CD4mut
  • L3T4
  • Ly-4
  • p55
  • W3/25
Alternatives
anti-Human CD4 antibody for Flow Cytometry
Reactivity
Human
1160
652
237
72
55
41
25
23
22
21
19
15
15
15
11
10
9
8
7
7
6
5
5
5
3
2
2
2
1
1
1
Host
Mouse
1328
624
269
8
4
1
Clonality (Clone)
Monoclonal ()
Conjugate
This CD4 antibody is un-conjugated
274
237
170
106
45
44
42
35
35
35
33
33
24
22
21
20
20
18
17
17
17
17
16
15
13
13
12
8
8
7
7
7
6
4
4
4
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Application
Functional Studies (Func), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))
1742
425
400
333
304
299
181
137
115
67
33
28
28
27
22
18
14
14
11
9
7
6
6
6
5
4
3
2
2
2
2
2
2
2
1
1
1
1
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Immunogen Human PHA blasts. Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
Clone RPA-T4
Isotype IgG1
Specificity This antibody recognises CD4. Epitope mapping studies have shown that clone RPA-T4 recognises an epitope within domain 1 of the extracellular region of the CD4 Molecule. Clone RPA-T4 has been reported to block gp120-CD4 interaction and inhibit syncytium formation (See Voehringer, D. et al. for details).
Purification Affinity Chromatography on Protein G
Endotoxin Level Low Endotoxin
Alternative Name CD4 (CD4 Antibody Abstract)
Background CD4 is a 55kD cell surface glycoprotein that is expressed primarily on a subpopulation of T lymphocytes and on peripheral blood monocytes and tissue macrophages.Synonyms: T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4
Gene ID 920
NCBI Accession NP_000607
UniProt P01730
Research Area Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells
Pathways TCR Signaling
Application Notes Functional Assays. Flow Cytometry: 1/50 - 1/100, Use 10 μL of the suggested working dilution to label 10^6 cells or 100 μL whole blood. Immunohistochemistry on frozen sections: 1/200 - 1/2000. Recommended Positive Control: Human lymphatic tissue
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer PBS, pH 7.4, None, None
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Upon receipt, store undiluted (in aliquots) at -20 °C.
Background publications Zhang, de Lara, Worth, Hegedus, Laamanen, Beverley, Macallan: "Accelerated in vivo proliferation of memory phenotype CD4+ T-cells in human HIV-1 infection irrespective of viral chemokine co-receptor tropism." in: PLoS pathogens, Vol. 9, Issue 4, pp. e1003310, 2013 (PubMed).

Voehringer, Koschella, Pircher: "Lack of proliferative capacity of human effector and memory T cells expressing killer cell lectinlike receptor G1 (KLRG1)." in: Blood, Vol. 100, Issue 10, pp. 3698-702, 2002 (PubMed).

Zarkesh-Esfahani, Pockley, Metcalfe, Bidlingmaier, Wu, Ajami, Weetman, Strasburger, Ross: "High-dose leptin activates human leukocytes via receptor expression on monocytes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 167, Issue 8, pp. 4593-9, 2001 (PubMed).

Wright, Jones, Puklavec, Brown, Barclay: "The unusual distribution of the neuronal/lymphoid cell surface CD200 (OX2) glycoprotein is conserved in humans." in: Immunology, Vol. 102, Issue 2, pp. 173-9, 2001 (PubMed).

Clevers, Alarcon, Wileman, Terhorst: "The T cell receptor/CD3 complex: a dynamic protein ensemble." in: Annual review of immunology, Vol. 6, pp. 629-62, 1988 (PubMed).

Clevers, Dunlap, Terhorst: "The transmembrane orientation of the epsilon chain of the TcR/CD3 complex." in: European journal of immunology, Vol. 18, Issue 5, pp. 705-10, 1988 (PubMed).

Campana, Thompson, Amlot, Brown, Janossy: "The cytoplasmic expression of CD3 antigens in normal and malignant cells of the T lymphoid lineage." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 138, Issue 2, pp. 648-55, 1987 (PubMed).

Erber, Pinching, Mason: "Immunocytochemical detection of T and B cell populations in routine blood smears." in: Lancet, Vol. 1, Issue 8385, pp. 1042-6, 1984 (PubMed).

Clark, Martin, Hansen, Ledbetter: "Evolution of epitopes on human and nonhuman primate lymphocyte cell surface antigens." in: Immunogenetics, Vol. 18, Issue 6, pp. 599-615, 1984 (PubMed).

Meuer, Acuto, Hussey, Hodgdon, Fitzgerald, Schlossman, Reinherz: "Evidence for the T3-associated 90K heterodimer as the T-cell antigen receptor." in: Nature, Vol. 303, Issue 5920, pp. 808-10, 1983 (PubMed).

Beverley, Callard: "Distinctive functional characteristics of human "T" lymphocytes defined by E rosetting or a monoclonal anti-T cell antibody." in: European journal of immunology, Vol. 11, Issue 4, pp. 329-34, 1981 (PubMed).

Kung, Goldstein, Reinherz, Schlossman: "Monoclonal antibodies defining distinctive human T cell surface antigens." in: Science (New York, N.Y.), Vol. 206, Issue 4416, pp. 347-9, 1979 (PubMed).