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CD45 antibody (Protein tyrosine Phosphatase, Receptor Type, C)

Details for Product anti-PTPRC Antibody No. ABIN119544, Supplier: Log in to see
Antigen
  • B220
  • CD45
  • Cd45
  • cd45
  • CD45R
  • GP180
  • L-CA
  • Lca
  • LCA
  • loc
  • Ly-5
  • LY5
  • Lyt-4
  • PTPRC
  • RT7
  • si:dkeyp-47c5.1
  • T200
Reactivity
Human
1201
727
207
135
62
51
41
39
23
21
17
8
5
5
4
3
3
2
1
1
1
1
1
1
Host
Mouse
1278
782
217
2
2
Clonality (Clone)
Monoclonal ()
Conjugate
This CD45 antibody is un-conjugated
280
228
168
98
53
52
49
40
35
35
30
27
27
25
24
24
24
24
22
17
17
17
14
14
13
13
10
10
9
7
6
6
6
6
6
6
5
5
5
4
4
3
3
3
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
1852
525
499
472
428
372
325
227
191
50
46
40
26
16
11
10
7
7
6
6
4
4
2
2
2
2
2
2
2
2
2
1
1
Supplier
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Immunogen Human T lymphocytes
Clone F8-11-13
Isotype IgG1
Specificity Recognises the human CD45RA cell surface antigen. CD45 is a complex molecule existing in a number of isoforms. Antibodies recognising a common epitope on all of these isoforms are termed CD45 whilst those recognising only individual isoforms are termed CD45RA or CD45RO etc. Clone F8-11-13 reacts with the high molecular weight form of the leucocyte common antigen (LCA) expressed by B lymphocytes and a subset of T lymphocytes. This product is routinely tested in flow cytometry on human peripheral blood lymphocytes.
Purification Protein G affinity purified immunoglobulin fraction
Alternative Name CD45 / LCA (PTPRC Antibody Abstract)
Background CD45 is a family of single chain transmembraneous glycoproteins consisting of at least four isoforms (220, 205, 190, 180 kDa) which share a common large intracellular domain. Their extracellular domains are heavily glycosylated. The different isoforms are produced by alternative messenger RNA splicing of three exons of a single gene on chromosome 1. CD45 is expressed on cells of the human hematopoietic lineage (including hematopoietic stem cells) with the exception of mature red cells. It is not detected on differentiated cells of other tissues. It is likely that CD45 plays an important role in signal transduction, inhibition or upregulation of various immunological functions. Antibodies recognising a common epitope on all of the isoforms are termed CD45 whilst those recognising only individual isoforms are termed CD45RA or CD45RO etc.Synonyms: L-CA, Leukocyte common antigen, PTPRC, Receptor-type tyrosine-protein phosphatase C, T200
Gene ID 5788
NCBI Accession NP_002829
UniProt P08575
Application Notes Flow cytometry (Use 10 μL of 1/50 - 1/100 diluted antibody to label 10^6 cells in 100 μL). Immunohistochemistry on frozen and paraffin embedded sections (1/200, Does not require protein digestion pre-treatment nor antigen retrieval using heat treatment). Recommended Positive Control Tissue: Human tonsil.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer PBS buffer with 0.09 % sodium azide as preservative
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Do not freeze working dilutions
Background publications Franitza, Kollet, Brill, Vaday, Petit, Lapidot, Alon, Lider: "TGF-beta1 enhances SDF-1alpha-induced chemotaxis and homing of naive T cells by up-regulating CXCR4 expression and downstream cytoskeletal effector molecules." in: European journal of immunology, Vol. 32, Issue 1, pp. 193-202, 2001 (PubMed).

OGrady, Stewart, Lowrey, Howie, Krajewski: "CD40 expression in Hodgkin's disease." in: The American journal of pathology, Vol. 144, Issue 1, pp. 21-6, 1994 (PubMed).