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Insulin Receptor Substrate 1 (IRS1) (AA 298-316), (pSer307) antibody

Details for Product No. ABIN129580, Supplier: Log in to see
Antigen
  • irs1
  • irsu
  • IRS1
  • HIRS-1
  • G972R
  • IRS-1
  • IRS1IRM
  • irs-1
  • irs1-a
Alternatives
anti-Human Insulin Receptor Substrate 1 antibody for Enzyme Immunoassay
Epitope
AA 298-316, pSer307
54
49
44
44
24
23
21
21
16
16
15
14
13
13
13
13
13
13
9
9
8
7
6
5
5
4
4
4
4
3
3
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
513
354
337
11
9
5
1
1
Host
Rabbit
510
30
Clonality
Polyclonal
Conjugate
Un-conjugated
13
12
11
11
11
10
10
10
10
10
10
10
2
1
1
1
Application
ELISA, Western Blotting (WB)
312
215
136
112
102
79
30
30
12
4
2
1
Supplier
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Immunogen This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 298-316 of human IRS1 protein.
Isotype IgG
Characteristics Concentration Definition: by UV absorbance at 280 nm
Alternative Name IRS1 (IRS1 Antibody Abstract)
Background Insulin Receptor Substrate 1 (IRS1) acts as a signaling molecule for IL-4, insulin and insulin-like growth factor-I (IGF-I) receptors.  When phosphorylated by the insulin receptor, IRS1 binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2.  When bound, IRS1 typically activates phos-phatidylinositol 3-kinase p85 subunit.  IRS1 interacts with both the NPXY motif of tyrosine-phos-phorylated IGF1R and the INSR through the PTB domain.  Serine phosphorylation of IRS1 is a mechanism for insulin resistance. Ser-312 phosphorylation inhibits insulin action through disruption of IRS1 interaction with the
Synonyms: HIRS 1 antibody, HIRS1 antibody, Insulin Receptor Substrate 1 antibody, IRS 1 antibody
Gene ID 3667
NCBI Accession NP_005535
UniProt P35568
Research Area Cardiovascular, Atherosclerosis, Signaling, Growth Factors, Cancer
Pathways Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
Application Notes This affinity purified antibody has been tested for use in ELISA and western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 130 kDa in size corresponding to phosphorylated IRS1 protein by western blotting in the appropriate cell lysate or extract.   Minimal reactivity is observed against the non-phosphorylated form of the immunizing peptide.  This antibody is phospho specific for pS307 of IRS1 protein.
Restrictions For Research Use only
Format Liquid
Concentration 0.76 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
Western Blotting (WB) image for anti-Insulin Receptor Substrate 1 (IRS1) (AA 298-316), (pSer307) antibody (ABIN129580) Western blot using Affinity Purified anti-IRS1 pS307 antibody shows detection of a ba...
Product cited in: Shah, Hunter: "Turnover of the active fraction of IRS1 involves raptor-mTOR- and S6K1-dependent serine phosphorylation in cell culture models of tuberous sclerosis." in: Molecular and cellular biology, Vol. 26, Issue 17, pp. 6425-34, 2006 (PubMed).