CD4 antibody (PE)
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- Target See all CD4 Antibodies
- CD4
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Reactivity
- Rat
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This CD4 antibody is conjugated to PE
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Application
- Flow Cytometry (FACS)
- Specificity
- Rat CD4
- Purification
- Purified from ascitic fluid via Protein G Chromatography.
- Immunogen
- Rat thymocyte membrane . Fusion Partner: P3-NSI-1-Ag 4 (NSI/1) . Donor: BALB/c spleen
- Clone
- W3-25
- Isotype
- IgG1
- Top Product
- Discover our top product CD4 Primary Antibody
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- Restrictions
- For Research Use only
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- Concentration
- 0.1 mg/ml
- Buffer
- R-PE conjugated Ig buffered in PBS, 0.02% NaN and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml. (Purified from ascitic fluid via Protein G Chromatography).
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C
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- Target
- CD4
- Alternative Name
- CD4 (CD4 Products)
- Synonyms
- cd4 antibody, L3T4 antibody, Ly-4 antibody, CD4mut antibody, W3/25 antibody, p55 antibody, CD4 molecule antibody, carcinoembryonic antigen-related cell adhesion molecule 5 antibody, T-cell surface glycoprotein CD4 antibody, CD4 antigen antibody, Cd4 molecule antibody, CD4 antibody, LOC100125525 antibody, cd4 antibody, Cd4 antibody
- Background
- Anti-rat CD4 monoclonal antibody recognizes a determinant on the majority of thymocytes (90-95%), a subset of peripheral T cells and peritoneal macrophages. (1,2,3,10.) The antigen recognized by this antibody is a surface glycoprotein of Mr 48,000-52,000 and is the homologue of the human CD4 and the mouse L3/T4 antigen. ABIN133182labels the rat T helper subset, which mediates the helper activity for B and T cells, graft vs. host (GVH) reactivity and produces IL-2 in the mixed lymphocyte reaction (MLR). (2,4,6,). ABIN133182is invaluable for separating T cell subsets for functional studies. It has been used in studying the role of T lymphocytes in graft rejections (7) and in studying the subsets of T cells in the rat which mediate graft vs. host disease .(8) This particular antibody is also one of three antibodies which labels T lymphocyte populations in the rat. These clones include W3/13 ABIN133182, which labels all T cells, as well as MRC OX-8 ABIN133182 and W3/25 ABIN133182 which label non- overlapping T cell subpopulations. These monoclonal antibodies used in concert are being employed extensively to investigate cellular aspects of the immune response in rats and prove to be useful as markers for functionally distinct subpopulations of lymphocytes.
- Pathways
- TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events
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