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Details for Product No. ABIN135139

Integrin alpha M (ITGAM) antibody (RPE)

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Antigen
Synonyms CR3, CR3A, MAC1, Cd11b, Ly-40, Mac-1, Mac-1a, CD11b/CD18, F730045J24Rik, CD11B, MAC-1, MAC1A, MO1A, SLEB6, C3bi
Reactivity
»Alternatives Mouse (Murine)
Host
Clonality (Clone) Monoclonal ()
Conjugate
Application
»Alternatives Flow Cytometry (FACS), Immunohistochemistry (IHC)
Pubmed 4 references available
Catalog no. ABIN135139
Quantity 0.1 mg
Price
187.00 $   Plus shipping costs $45.00
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Immunogen C57BL/10 mouse splenic T cells and concanavaline A-activated C57BL/10 splenocytes1
Clone M1-70
Isotype IgG2b
Characteristics The antibody detects: M subunit (Mr 170 kDa) of the CD11b/CD18 heterodimer
Cross-reaction of M1/70 to human CD11b has been observed on monocytes, polymorphonuclear leukocytes, and NK cells.
Alternative Name CD11b/Mac-1a
Background CD11b, the integrin M subunit, combines with CD18, the integrin 2 subunit, to form the integrin Mac-1, also known as complement receptor 3 (CR3), which mediates adhesion to C3bi and ICAM-1/CD54. Mac-1 is expressed at varying levels on granulocytes, macrophages, dendritic cells, NK cells, and B-1 cells in the peritoneal and pleural cavities. Mac-1 expression is rapidly up-regulated on neutrophils after activation and in the same time period that CD62L is shed from the cell surface. In addition to its role in binding C3bi on opsonized targets and mediation of the subsequent ingestion process, Mac-1 is important as an adhesion molecule in the transendothelial migration of monocytes and neutrophils.
Application Notes Working Dilution:
FACS: 03 µg/10^6 cells
Representative data are included in this product insert.

Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
Comment

In vitro and in vivo blocking

Sample Volume 1 mL
Restrictions For Research Use only
Buffer Product is supplied as 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL of PBS / NaN3 and a stabilizing agent.
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Do not freeze!
Protect conjugated products from light.

Each reagent is stable for the period shown on the bottle label if stored as directed.
Storage 4 °C
Springer, Galfrè, Secher et al.: "Monoclonal xenogeneic antibodies to murine cell surface antigens: identification of novel leukocyte differentiation antigens." in: European journal of immunology, Vol. 8, Issue 8, pp. 539-51, 1978 (PubMed).

Flotte, Springer, Thorbecke: "Dendritic cell and macrophage staining by monoclonal antibodies in tissue sections and epidermal sheets." in: The American journal of pathology, Vol. 111, Issue 1, pp. 112-24, 1983 (PubMed).

Springer: "Traffic signals for lymphocyte recirculation and leukocyte emigration: the multistep paradigm." in: Cell, Vol. 76, Issue 2, pp. 301-14, 1994 (PubMed).

Gyetko, Sud, Kendall et al.: "Urokinase receptor-deficient mice have impaired neutrophil recruitment in response to pulmonary Pseudomonas aeruginosa infection." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 165, Issue 3, pp. 1513-9, 2000 (PubMed).

Alternatives for antigen "Integrin alpha M (ITGAM)", type "Antibodies"
Hosts (155), (124), (30), (4), (2)
Reactivities (156), (125), (25), (9), (6), (5), (4), (3), (3), (3), (1), (1), (1), (1)
Applications (251), (70), (44), (41), (36), (33), (28), (26), (13), (11), (9), (3), (3), (2), (1), (1), (1)
Conjugates (44), (32), (32), (10), (9), (8), (4), (4), (3), (2), (2), (2), (1), (1)
Epitopes (3), (3), (1), (1), (1), (1), (1), (1)
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