SF3B1 antibody
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- Target See all SF3B1 Antibodies
- SF3B1 (Splicing Factor 3b, Subunit 1, 155kDa (SF3B1))
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Reactivity
- Human, Mouse, Hamster
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This SF3B1 antibody is un-conjugated
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Application
- Western Blotting (WB)
- Specificity
- This antibody reacts with Sap155.
- Cross-Reactivity (Details)
- Species reactivity (tested):Human, Mouse, Hamster
- Purification
- Protein A agarose
- Immunogen
- Recombinant mouse Sap155
- Clone
- 1A5
- Isotype
- IgG2b
- Top Product
- Discover our top product SF3B1 Primary Antibody
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL
- Buffer
- PBS containing 50 % glycerol, pH 7.2. No preservative is contained.
- Preservative
- Without preservative
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- Target
- SF3B1 (Splicing Factor 3b, Subunit 1, 155kDa (SF3B1))
- Alternative Name
- SF3B1 / SAP155 (SF3B1 Products)
- Synonyms
- Cus1 antibody, SAP145 antibody, SF3B145 antibody, SF3b1 antibody, SF3b150 antibody, Hsh155 antibody, MDS antibody, PRP10 antibody, PRPF10 antibody, SAP155 antibody, SF3b155 antibody, 155kDa antibody, 2810001M05Rik antibody, AA409119 antibody, Prp10 antibody, TA-8 antibody, Targ4 antibody, hsh155 antibody, prp10 antibody, prpf10 antibody, sap155 antibody, SF3B1 antibody, Sap155 antibody, wu:fb99f09 antibody, splicing factor 3b subunit 2 antibody, splicing factor 3b subunit 1 antibody, splicing factor 3b, subunit 1 antibody, splicing factor 3b subunit 1 S homeolog antibody, SF3B2 antibody, SF3B1 antibody, Sf3b1 antibody, sf3b1.S antibody, sf3b1 antibody
- Background
- SF3 is a U2 snRNP-associated protein complex essential for spliceosome assembly and splicing catalysis of the major spliceosome. SF3 contains the Spliceosome-Associated Proteins, SAP 49, 130, 145, and 155. SAP155/Sf3b1 is an essential subunit of the U2 snRNP for mRNA splicing and has also been identified in the minor (U12-dependent) spliceosome. SAP155 interacts with the mammalian PcG (Polycomb group) proteins, Mel18 and Ring1B by the yeast two-hybrid system. SAP155 contains numerous Cdk consensus phosphorylation sites in its N terminus and is phosphorylated prior to catalytic step II of the splicing pathway. SAP155 serves as a substrate for cyclin E-cdk2 in vitro, suggesting that pre-mRNA splicing may be linked to the cell cycle machinery in mammalian cells.Synonyms: Pre-mRNA-splicing factor SF3b 155 kDa subunit, SAP 155, SAP-155, SF3b155, Spliceosome-associated protein 155, Splicing factor 3B subunit 1
- Gene ID
- 23451
- NCBI Accession
- NP_036565
- UniProt
- O75533
- Pathways
- Chromatin Binding
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