SIRT1 antibody (AA 577-590)
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- Target See all SIRT1 Antibodies
- SIRT1 (Sirtuin 1 (SIRT1))
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Binding Specificity
- AA 577-590
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Reactivity
- Human, Monkey
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- This SIRT1 antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Brand
- IHC-plus™
- Specificity
- Human SIRT1. This antibody is expected to recognize both reported isoforms (NP_036370.2, NP_001135970.1).
- Predicted Reactivity
- Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset (100%) Mouse (93%) Rat (86%).
- Purification
- Immunoaffinity purified
- Immunogen
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Synthetic peptide C-EKPQEVQTSRNVES from an internal region of human SIRT1 (NP_036370.2, NP_001135970.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset (100%), Mouse (93%), Rat (86%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product SIRT1 Primary Antibody
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- Application Notes
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Approved: IHC, IHC-P (3.75 μg/mL), Peptide-ELISA (1:64000), WB (0.05 - 0.2 μg/mL)
Usage: Peptide ELISA: antibody detection limit dilution 1:64000. Western blot: Approx 120kD band observed in lysates of cell line Jurkat (calculated MW of 81.7kD according to NP_036370.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Cheng et al, Proc Natl Acad Sci U S A. 2003 Sep 16,100(19):10794-9., PMID: 12960381). Recommended concentration: 0.05-0.2 μg/mL. - Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- Tris-buffered saline, pH 7.3, 0.5 % BSA, 0.02 % sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Minimize freezing and thawing.
- Storage
- -20 °C
- Storage Comment
- Store at -20°C. Avoid freeze-thaw cycles.
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- Target
- SIRT1 (Sirtuin 1 (SIRT1))
- Alternative Name
- SIRT1 / Sirtuin 1 (SIRT1 Products)
- Synonyms
- sirtuin 1 antibody, AA673258 antibody, SIR2L1 antibody, Sir2 antibody, Sir2a antibody, Sir2alpha antibody, sir2l1 antibody, sirtuin antibody, SIR2 antibody, sirtuin 1 antibody, sirtuin 1 L homeolog antibody, SIRT1 antibody, SRT1 antibody, Sirt1 antibody, sirt1.L antibody
- Background
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Name/Gene ID: SIRT1
Family: ADP-Ribosyltransferase
Synonyms: SIRT1, SIR2-like protein 1, SIR2alpha, SIR2L1, Sirtuin 1, Sir2-like 1, Sirtuin type 1, HSIR2, HSIRT1 - Gene ID
- 23411
- NCBI Accession
- NP_036370, NP_001135970
- UniProt
- Q96EB6
- Pathways
- MAPK Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Carbohydrate Homeostasis, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus, Negative Regulation of intrinsic apoptotic Signaling
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