EIF4EBP1 antibody (Eukaryotic Translation Initiation Factor 4E Binding Protein 1) (pThr69)

Details for Product anti-EIF4EBP1 Antibody No. ABIN1531251, Supplier: Log in to see
Antigen
  • 153432_at
  • 43-BP
  • 4E-BP
  • 4E-BP1
  • 4EBP
  • 4e-bp
  • 4eBP
  • 4ebp
  • BcDNA.HL08053
  • BcDNA:HL08053
  • CG8846
  • CT8705
  • Dm 4E-BP
  • Dmel\\CG8846
  • Phas1
  • Thor/EIF4EBP
  • anon-WO0172774.158
  • d4E-BP
  • d4E-BP1
  • d4EBP
  • d4e-bp
  • d4eBP
  • dthor
  • eIF4E-BP
  • l(2)06270
  • l(2)k07736
  • l(2)k13517
  • pp20
  • thor
  • EIF4EBP1
  • fc04h09
  • wu:fc04h09
  • zgc:64137
  • 4EBP1
  • BP-1
  • PHAS-I
  • 4e-bp1
  • AA959816
  • CG8846 gene product from transcript CG8846-RA
  • eukaryotic translation initiation factor 4E binding protein 1
  • Thor
  • EIF4EBP1
  • eif4ebp1
  • LOC100230789
  • Eif4ebp1
Alternatives
anti-Human EIF4EBP1 antibody for Western Blotting
Epitope
pThr69
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36
30
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21
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16
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15
15
12
12
8
8
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5
3
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3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Mouse (Murine), Rat (Rattus)
462
277
235
20
17
13
9
5
5
5
3
2
2
1
1
1
1
1
Host
Rabbit
401
97
4
Clonality
Polyclonal
Conjugate
This EIF4EBP1 antibody is un-conjugated
19
17
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8
5
5
5
5
5
5
5
5
5
5
5
1
1
1
1
1
1
1
1
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1
1
1
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
345
215
200
111
66
30
18
17
14
8
7
6
5
4
3
1
Options
Supplier
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Immunogen The antiserum was produced against synthesized peptide derived from mouse 4E-BP1 around the phosphorylation site of Thr69.
Isotype IgG
Specificity 4E-BP1 (Phospho-Thr69) Antibody detects endogenous levels of 4E- BP1 only when phosphorylated at Thr69.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation.
Purity > 95 %
Alternative Name 4E-BP1 (EIF4EBP1 Antibody Abstract)
Background Molecular weight of target protein: 12 kDa
Synonyms: 4EBP1, EIF4EBP1, Eukaryotic translation initiation factor 4E binding protein 1, Insulin-stimulated EIF-4E binding protein PHAS-I, P/OKCL.6, PHAS-1, PHAS-I
Molecular Weight 12 kDa
Gene ID 13685
UniProt Q60876
Pathways MAPK Signaling, PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, Regulation of Cell Size
Application Notes WB: 1:500-1:1000
IHC: 1:50-1:100
ELISA: 1:5000
Optimal working dilution should be determined by the investigator.
Comment

Unigene-Number: Mm.6700 (NCBI Gene Symbol: EIF4EBP1)

Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 52 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Stable at -20 °C for at least 1 year.
Expiry Date 12 months
Supplier Images
Western Blotting (WB) image for anti-Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1) (pThr69) antibody (ABIN1531251) Western blot analysis of extracts from Jurkat cells treated with EGF 200ng/ml 30', us...
Immunohistochemistry (IHC) image for anti-Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1) (pThr69) antibody (ABIN1531251) Immunohistochemistry analysis of paraffin-embedded human brain carcinoma, using 4E-BP...
Background publications Pause, Belsham, Gingras, Donzé, Lin, Lawrence, Sonenberg: "Insulin-dependent stimulation of protein synthesis by phosphorylation of a regulator of 5'-cap function." in: Nature, Vol. 371, Issue 6500, pp. 762-7, 1994 (PubMed).

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