Metabotropic Glutamate Receptor 5 antibody (C-Term)

Catalog No. ABIN1574147

Product Details anti-Metabotropic Glutamate Receptor 5 Antibody

Target: Metabotropic Glutamate Receptor 5 (GRM5) (Glutamate Receptor, Metabotropic 5 (GRM5))

Binding Specificity: C-Term

Reactivity: Rat, Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Metabotropic Glutamate Receptor 5 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Specificity: Rabbit Anti-mGluR5 Polyclonal Antibody detects endogenous levels of human mGluR5. It is predicted to react with mouse and rat mGluR5 protein according to sequence homology.Positive Control: Ramos

Cross-Reactivity (Details): Rabbit Anti-mGluR5 Polyclonal Antibody detects endogenous levels of human mGluR5. It is predicted to react with mouse and rat mGluR5 protein according to sequence homology.Positive Control: Ramos

Purification: Immunoaffinity chromatography

Immunogen: KLH-coupled synthetic peptide from C-terminal of human mGluR5

Isotype: IgG

Application Details

Application Notes: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Western blot: 1-2 µg/mLFlow cytometry: 1-3 µg for 1 x 106 cellsOther applications: user-optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: PBS, pH 7.4, containing 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Storage: 4 °C/-20 °C

Storage Comment: The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Target Details for Metabotropic Glutamate Receptor 5

Target: Metabotropic Glutamate Receptor 5 (GRM5) (Glutamate Receptor, Metabotropic 5 (GRM5))

Alternative Name: mGluR5 (GRM5 Products)

Synonyms: GPRC1E antibody, MGLUR5 antibody, mGlu5 antibody, mGluR5 antibody, mGlur5 antibody, 6430542K11Rik antibody, AI850523 antibody, Glu5R antibody, Gprc1e antibody, mGluR5b antibody, glutamate receptor, metabotropic 5 antibody, glutamate metabotropic receptor 5 antibody, GRM5 antibody, Grm5 antibody

Background: Metabotropic glutamate receptor 5 (mGluR5) is one member of G-protein coupled receptor 3 family. The activity of mGluR5 receptor is mediated by a G-protein that activates phosphatidylinositol-calcium second messenger system and generates calcium-activated chloride current. mGluR5 plays a critical role in the modulation of synaptic plasticity and behavioral responses to multiple substances of abuse. The expression of mGluR5 is widely distributed in brain regions relating to memory, such as the hippocampus. mGluR5 regulates phospholipid hydrolysis in developing cortical neurons through the activation of PLC-beta1. The receptor regulates glutamatergic transmission in a variety of brain regions, particularly those thought to participate in reward-related behaviors. Its activation by the group I mGluR agonist (RS)-3,5-dihydroxyphenylglycine (DHPG) can induce long term depression (DHPG-LTD) of glutamatergic synaptic transmission.Rabbit Anti-mGluR5 Polyclonal Antibody is developed in rabbit using a KLH-coupled synthetic peptide from C-terminal of human mGluR5 (Swiss Prot: P41594).

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling, Regulation of long-term Neuronal Synaptic Plasticity