GFP antibody (Green Fluorescent Protein) (AA 183-191)

Details for Product anti-GFP Antibody No. ABIN1742292, Supplier: Log in to see
Antigen
  • green fluorescent protein
  • gfp
Epitope
AA 183-191
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Reactivity
Aequorea victoria
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Host
Mouse
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Clonality (Clone)
Monoclonal ()
Conjugate
This GFP antibody is un-conjugated
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Application
Western Blotting (WB)
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Options
Supplier
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Supplier Product No.
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Immunogen Recombinant full length GFP, glutaraldehyde fixed. Epitope QQNTPIGDG (aa 183-191)
Clone 101G4
Isotype IgG2b
Specificity Specific for GFP of jelly-fish.
Purification purified IgG. Azide was added before lyophilization.
Alternative Name GFP
Research Area Tags/Labels
Application Notes WB: 1 : 1000 up to 1 : 10 000 (AP staining)
IP: no (methanol fixated material only)
IHC: not tested yet
Comment

ABIN1742292 is recommended for IP and ICC.

Restrictions For Research Use only
Format Lyophilized
Reconstitution For reconstitution add 100 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
Buffer PBS, 0.02% sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1 mg/mL protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.
Storage -20 °C
Storage Comment Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.
Supplier Images
Western Blotting (WB) image for anti-Green Fluorescent Protein (GFP) (AA 183-191) antibody (ABIN1742292) dilution: 1 : 1000, sample: recombinant protein
Product cited in: Koestler, Steffen, Nemethova, Winterhoff, Luo, Holleboom, Krupp, Jacob, Vinzenz, Schur, Schlüter, Gunning, Winkler, Schmeiser, Faix, Stradal, Small, Rottner: "Arp2/3 complex is essential for actin network treadmilling as well as for targeting of capping protein and cofilin." in: Molecular biology of the cell, Vol. 24, Issue 18, pp. 2861-75, 2013 (PubMed).

Oelkers, Vinzenz, Nemethova, Jacob, Lai, Block, Szczodrak, Kerkhoff, Backert, Schlüter, Stradal, Small, Koestler, Rottner: "Microtubules as platforms for assaying actin polymerization in vivo." in: PLoS ONE, Vol. 6, Issue 5, pp. e19931, 2011 (PubMed).

Kaddai, Gonzalez, Keslair, Grémeaux, Bonnafous, Gugenheim, Tran, Gual, Le Marchand-Brustel, Cormont: "Rab4b is a small GTPase involved in the control of the glucose transporter GLUT4 localization in adipocyte." in: PLoS ONE, Vol. 4, Issue 4, pp. e5257, 2009 (PubMed).

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