GAD65 antibody (AA 3-96)
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- Target See all GAD65 (GAD2) Antibodies
- GAD65 (GAD2) (Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2))
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Binding Specificity
- AA 3-96
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Reactivity
- Rat, Mouse
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This GAD65 antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunocytochemistry (ICC)
- Specificity
- Specific for GAD 2 / GAD 65.
- Purification
- Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.
- Immunogen
- Purified recombinant mouse GAD 2 (aa 3-96).
- Top Product
- Discover our top product GAD2 Primary Antibody
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- Application Notes
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WB: 1 : 1000 (AP staining)
IP: not tested yet
ICC: 1 : 2000
IHC: 1 : 1000 - Comment
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ICC/IHC: The crude antiserum is recommended. ELISA: Suitable as detector antibody for sandwich-ELISA with ABIN1742417 as capture antibody (protocol for sandwich-ELISA).
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
- Buffer
- PBS
- Handling Advice
- Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.
- Storage
- -20 °C
- Storage Comment
- Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!
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Early GABAergic transmission defects in the external globus pallidus and rest/activity rhythm alteration in a mouse model of Huntington's disease." in: Neuroscience, Vol. 329, pp. 363-79, (2016) (PubMed).
: "Blocking L-type voltage-gated Ca2+ channels with dihydropyridines reduces gamma-aminobutyric acid type A receptor expression and synaptic inhibition." in: The Journal of biological chemistry, Vol. 284, Issue 47, pp. 32544-50, (2009) (PubMed).
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Early GABAergic transmission defects in the external globus pallidus and rest/activity rhythm alteration in a mouse model of Huntington's disease." in: Neuroscience, Vol. 329, pp. 363-79, (2016) (PubMed).
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- Target
- GAD65 (GAD2) (Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2))
- Alternative Name
- GAD 2 (GAD2 Products)
- Synonyms
- GAD65 antibody, gad65 antibody, F12P19.12 antibody, F12P19_12 antibody, GLUTAMATE DECARBOXYLASE 2 antibody, glutamate decarboxylase 2 antibody, zgc:112198 antibody, 6330404F12Rik antibody, GAD(65) antibody, Gad-2 antibody, glutamate decarboxylase 2 antibody, glutamate decarboxylase 1 antibody, glutamic acid decarboxylase 2 antibody, GAD2 antibody, PTRG_06148 antibody, LOC9324534 antibody, Gad2 antibody, GAD1 antibody, gad2 antibody
- Background
- Synonyms: GAD 65
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