RNF7 antibody (AA 59-71)

Catalog No. ABIN1804120

Product Details anti-RNF7 Antibody

Target: RNF7 (Ring Finger Protein 7 (RNF7))

Binding Specificity: AA 59-71

Reactivity: Human, Mouse, Rat, Dog, Horse, Monkey, Pig, Bat, Xenopus laevis, Chicken, Zebrafish (Danio rerio), Cow

Host: Goat

Clonality: Polyclonal

Conjugate: This RNF7 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human RNF7. This antibody is expected to recognize isoform 1 (NP_055060.1).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide DACLRCQAENKQE from an internal region of human RNF7 (NP_055060.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bat, Bovine, Horse, Pig, Opossum, Turkey, Chicken, Platypus, Lizard, Xenopus, Trout, Catfish, Salmon, Sablefish, Stickleback, Pufferfish, Zebrafish (100%), Tick (92%), Eye worm, Seq squirt, Sea anemone (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), Peptide-ELISA, WB (1 - 3 μg/mL)

Usage: Western Blot: Approx 11kD band observed in Human Human Heart lysates (calculated MW of 12.7kD according to NP_055060.1). Recommended concentration: 1-3 μg/mL. An additional band of 28kD was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when used for other assays than Western blot.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for RNF7

Target: RNF7 (Ring Finger Protein 7 (RNF7))

Alternative Name: RNF7 (RNF7 Products)

Synonyms: CKBBP1 antibody, ROC2 antibody, SAG antibody, Rbx2 antibody, ring finger protein 7 antibody, RNF7 antibody, Rnf7 antibody

Background: Name/Gene ID: RNF7

Synonyms: RNF7, CKII beta-binding protein 1, CKBBP1, RING-box protein 2, Rbx2, Regulator of cullins 2, Ring finger protein 7, ROC2, Zinc RING finger protein SAG

Gene ID: 9616

NCBI Accession: NP_055060

UniProt: Q9UBF6

Pathways: Positive Regulation of Endopeptidase Activity