MEK1 antibody (pSer221)

Catalog No. ABIN1842366

Product Details anti-MEK1 Antibody

Target: MEK1 (MAP2K1) (Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1))

Binding Specificity: pSer221

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MEK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Specificity: Rabbit Anti-MEK1 (Phospho-Ser221) Polyclonal Antibody detects endogenous levels of MEK1 only when phosphorylated at serine 221.

Characteristics: Molecular Weight: Predicted Band Size 43 kDa, Observed Band Size 43 kDa

Purification: The antibody is affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide is removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

Immunogen: Synthesized phosphopeptide derived from human MEK1 around pSer221 (A-N-SP-F-V)

Isotype: IgG

Application Details

Application Notes: Western blot: 1:500-1:1,000,
IHC: 1:50-1:100,
IF: 1:100-1:200

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide, and 50 % glycerol

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage.
Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20 °C for 1 year.

Target Details for MEK1

Target: MEK1 (MAP2K1) (Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1))

Alternative Name: MEK1 (MAP2K1 Products)

Synonyms: Mek1 antibody, CFC3 antibody, MAPKK1 antibody, MEK1 antibody, MKK1 antibody, PRKMK1 antibody, MEKK1 antibody, Prkmk1 antibody, mek-2 antibody, si:ch211-242m18.2 antibody, wu:fj56a12 antibody, wu:fj61b01 antibody, zgc:56557 antibody, ATMEK1 antibody, F20B18.180 antibody, F20B18_180 antibody, MAP kinase/ ERK kinase 1 antibody, MITOGEN ACTIVATED PROTEIN KINASE KINASE antibody, MITOGEN ACTIVATED PROTEIN KINASE KINASE 1 antibody, NMAPKK antibody, MEK antibody, mitogen activated protein kinase kinase 1 antibody, mitogen-activated protein kinase kinase 1 antibody, mitogen-activated protein kinase kinase 1 L homeolog antibody, MAP kinase/ ERK kinase 1 antibody, Map2k1 antibody, MAP2K1 antibody, map2k1.L antibody, map2k1 antibody, MEK1 antibody

Pathways: MAPK Signaling, RTK Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, Autophagy, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling