FOXO1 antibody (pSer319)
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- Target See all FOXO1 Antibodies
- FOXO1 (Forkhead Box O1 (FOXO1))
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Binding Specificity
- pSer319
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Reactivity
- Human, Mouse, Rat
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This FOXO1 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
- Specificity
- Rabbit Anti-FKHR (Phospho-Ser319) Polyclonal Antibody detects endogenous levels of FKHR only when phosphorylated at serine 319.
- Characteristics
- Molecular Weight: Predicted Band Size 78-82 kDa, Observed Band Size 78-82 kDa
- Purification
- The antibody is affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide is removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
- Immunogen
- Synthesized phosphopeptide derived from human FKHR around pSer319 (T-S-SP-N-A)
- Isotype
- IgG
- Top Product
- Discover our top product FOXO1 Primary Antibody
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- Application Notes
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Western blot: 1:500-1:1,000,
IHC: 1:50-1:100,
IF: 1:100-1:200 - Restrictions
- For Research Use only
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- Concentration
- 1 mg/mL
- Buffer
- phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide, and 50 % glycerol
- Preservative
- Sodium azide
- Precaution of Use
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- Handling Advice
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Briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage.
Avoid repeated freezing and thawing. - Storage
- -20 °C
- Storage Comment
- Store at -20 °C for 1 year
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- Target
- FOXO1 (Forkhead Box O1 (FOXO1))
- Alternative Name
- FKHR (FOXO1 Products)
- Synonyms
- FKH1 antibody, FKHR antibody, FOXO1A antibody, FoxO1a.1 antibody, foxo1 antibody, zgc:153388 antibody, Fkhr antibody, Foxo1a antibody, AI876417 antibody, Afxh antibody, Fkhr1 antibody, fkh1 antibody, fkhr antibody, foxo1a antibody, xFoxO1 antibody, FoxO1A antibody, foxo1a.2 antibody, forkhead box O1 antibody, forkhead box O1 a antibody, forkhead box O1 L homeolog antibody, forkhead box O1 b antibody, FOXO1 antibody, foxo1a antibody, Foxo1 antibody, foxo1 antibody, foxo1.L antibody, foxo1b antibody
- Pathways
- PI3K-Akt Signaling, Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Carbohydrate Homeostasis, Chromatin Binding, Regulation of Carbohydrate Metabolic Process, CXCR4-mediated Signaling Events, BCR Signaling
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