SHC1 antibody (pTyr427)

Catalog No. ABIN1842516

Product Details anti-SHC1 Antibody

Target: SHC1 (SHC (Src Homology 2 Domain Containing) Transforming Protein 1 (SHC1))

Binding Specificity: pTyr427

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SHC1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Specificity: Rabbit Anti-Shc1 (Phospho-Tyr427) Polyclonal Antibody detects endogenous levels of Shc1 only when phosphorylated at tyrosine 427.

Characteristics: Molecular Weight: Predicted Band Size 63 kDa, Observed Band Size 63 kDa

Purification: Rabbit Anti-Shc1 (Phospho-Tyr427) Polyclonal Antibody is affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide is removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

Immunogen: Synthesized phosphopeptide derived from human Shc1 around pTyr427 (P-S-YP-V-N)

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:1,000,
IHC: 1:50-1:100,
IF: 1:100-1:200

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide, and 50 % glycerol

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage.,
Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store Rabbit Anti-Shc1 (Phospho-Tyr427) Polyclonal Antibody at -20 °C. It will remain stable for one year.

Target Details for SHC1

Target: SHC1 (SHC (Src Homology 2 Domain Containing) Transforming Protein 1 (SHC1))

Alternative Name: Shc1 (SHC1 Products)

Synonyms: SHC1 antibody, SHC antibody, SHCA antibody, P66shc antibody, si:dkey-246i14.4 antibody, wu:fc10h03 antibody, shc antibody, p66shc antibody, Shc antibody, ShcA antibody, p66 antibody, SHC adaptor protein 1 antibody, Shc1p antibody, squalene--hopene cyclase antibody, SHC (Src homology 2 domain containing) transforming protein 1 antibody, SHC (Src homology 2 domain containing) transforming protein 1 L homeolog antibody, src homology 2 domain-containing transforming protein C1 antibody, SHC1 antibody, shc1 antibody, Shc1 antibody, shc1.L antibody

Pathways: RTK Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, ER-Nucleus Signaling, Signaling Events mediated by VEGFR1 and VEGFR2