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Lymphocyte Cytosolic Protein 2 (SH2 Domain Containing Leukocyte Protein of 76kDa) (LCP2) (N-Term) antibody

Details for Product No. ABIN185252
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Antigen
Synonyms AI323664, BB161688, SLP-76, SLP76, twm, Slp76
Epitope
N-Term
(14), (12), (12), (9), (7), (5), (3), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(106), (41), (35), (17), (14), (14)
Host
Goat
(79), (20), (8), (2)
Clonality
Polyclonal
Conjugate
Un-conjugated
(3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB)
(79), (32), (30), (24), (22), (9), (2), (2), (2), (2), (1)
Pubmed 1 reference available
Quantity 100 µg
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Catalog No. ABIN185252
374.27 $
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Immunogen Peptide with sequence ALRNVPFRSEV-C, from the N Terminus of the protein sequence according to NP_005556.1.
Cross-Reactivity Mouse (Murine), Rat (Rattus), Dog (Canine), Pig (Porcine), Cow (Bovine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name SLP76 / LCP2
Background Synonyms: LCP2, SLP76, SLP-76, lymphocyte cytosolic protein 2 (SH2 domain containing leukocyte protein of 76kDa), 76 kDa tyrosine phosphoprotein, SH2 domain-containing leukocyte protein of 76kD, lymphocyte cytosolic protein 2 (SH2 domain containing leukocyte protein of 76kD)
Gene ID 3937
NCBI Accession NP_005556.1
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 75 kDa band observed in lysates of cell line Jurkat (calculated MW of 60.2 kDa according to NP_005556.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Jackman et al, J Biol Chem. 1995 Mar 31,270(13):7029-32. PMID: 7706237). Recommended concentration: 0.5-2 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-Lymphocyte Cytosolic Protein 2 (SH2 Domain Containing Leukocyte Protein of 76kDa) (LCP2) (N-Term) antibody ABIN185252 staining (0.5µg/ml) of Jurkat lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
General Jackman, Motto, Sun et al.: "Molecular cloning of SLP-76, a 76-kDa tyrosine phosphoprotein associated with Grb2 in T cells." in: The Journal of biological chemistry, Vol. 270, Issue 13, pp. 7029-32, 1995 (PubMed).

Validation Images
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