Forkhead Box O4 (FOXO4) (Internal Region) antibody

Details for Product No. ABIN190813
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Antigen
Synonyms FOXO4, foxo4, AFX, AFX1, MLLT7, Afxh, Mllt7, afx, RGD1561201
Epitope
Internal Region
(43), (26), (22), (19), (17), (7), (6), (5), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(213), (151), (107), (61), (48), (48), (36), (2), (1)
Host
Goat
(207), (5), (2)
Clonality
Polyclonal
Conjugate
Un-conjugated
(6), (6), (6), (5), (5), (5), (5), (5), (5), (5), (5), (1), (1), (1)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB)
(156), (72), (52), (50), (42), (29), (26), (1), (1)
Pubmed 1 reference available
Quantity 100 μg
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Catalog No. ABIN190813
374.27 $
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Immunogen Peptide with sequence C-EAASQDRMPQDLD, from the internal region of the protein sequence according to NP_005929.2.
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name FOXO4 / MLLT7
Background Synonyms: MLLT7, FOXO4, myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila) translocated to, 7, AFX, AFX1, MGC120490, myeloid/lymphoid or mixed-lineage leukemia (trithorax (Drosophila) homolog) translocated to, 7
Gene ID 4303
NCBI Accession NP_005929
Application Notes ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 60-65 kDa band observed in lysates of cell line K562 (calculated MW of 53.7 kDa according to NP_005929.2). Recommended concentration: 1-3 µg/mL. Enzyme immunoassay: Sandwich-type ELISA with increasing amount of recombinant FOXO4 captured by a rabbit antibody. Recommended reporter concentration: 0.5-1 µg/mL
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-Forkhead Box O4 (FOXO4) (Internal Region) antibody ABIN190813 (1µg/ml) staining of K562 cell lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
General Paik, Kollipara, Chu et al.: "FoxOs are lineage-restricted redundant tumor suppressors and regulate endothelial cell homeostasis." in: Cell, Vol. 128, Issue 2, pp. 309-23, 2007 (PubMed).

Validation Images
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