Retinoblastoma 1 antibody (RB1) (pSer807)

Details for Product anti-RB1 Antibody No. ABIN196841, Supplier: Log in to see
Antigen
  • RB1
  • OSRC
  • RB
  • p105-Rb
  • pRb
  • pp110
  • Rb
  • Rb-1
  • zgc:154147
  • P105-RB
  • PP110
  • RB11
  • retinoblastoma 1 (including osteosarcoma)
  • retinoblastoma 1
  • Retinoblastoma-associated protein
  • RB1
  • rb
  • Rb1
  • rb1
Alternatives
anti-Human Retinoblastoma 1 antibody for Immunohistochemistry (Frozen Sections)
Epitope
pSer807
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54
43
37
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29
20
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18
15
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14
8
8
8
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3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
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1
1
1
1
1
1
1
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Reactivity
Human
635
343
237
10
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5
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2
1
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Host
Rabbit
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1
Clonality
Polyclonal
Conjugate
This Retinoblastoma 1 antibody is un-conjugated
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7
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5
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3
2
1
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Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
395
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50
41
30
24
10
9
7
7
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2
1
1
1
Options
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Immunogen The antiserum was produced against synthesized phosphopeptide derived from human Rb around the phosphorylation site of serine 807 (Y-I-SP-P-L).
Specificity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. Rb (phospho-Ser807) antibody detects endogenous levels of Rb only when phosphorylated at serine 807.
Purification Immunoaffinity chromatography.
Alternative Name Retinoblastoma-Associated Protein / RB1 (RB1 Antibody Abstract)
Background Synonyms: OSRC, Rb, Retinoblastoma 1, p105-Rb, pRb, pp110
Gene ID 5925
NCBI Accession NP_000312
UniProt P06400
Pathways Cell Division Cycle, Intracellular Steroid Hormone Receptor Signaling Pathway, Mitotic G1-G1/S Phases, DNA Replication, Maintenance of Protein Location, Synthesis of DNA, Autophagy
Application Notes Suitable for use in Western blot (1: 500approx. 1: 1000) and Immunohistochemistry (1: 50approx. 1: 100).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store the antibody (in aliquots) at -20 °C.
Supplier Images
 image for anti-Retinoblastoma 1 (RB1) (pSer807) antibody (ABIN196841) anti-Retinoblastoma 1 (RB1) (pSer807) antibody
 image for anti-Retinoblastoma 1 (RB1) (pSer807) antibody (ABIN196841) anti-Retinoblastoma 1 (RB1) (pSer807) antibody (Image 2)
Background publications Roesch, Becker, Meyer, Hafner, Wild, Landthaler, Vogt: "Overexpression and hyperphosphorylation of retinoblastoma protein in the progression of malignant melanoma." in: Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, Vol. 18, Issue 4, pp. 565-72, 2005 (PubMed).

Chadee, Kyriakis: "MLK3 is required for mitogen activation of B-Raf, ERK and cell proliferation." in: Nature cell biology, Vol. 6, Issue 8, pp. 770-6, 2004 (PubMed).

Knudsen, Wang: "Dual mechanisms for the inhibition of E2F binding to RB by cyclin-dependent kinase-mediated RB phosphorylation." in: Molecular and cellular biology, Vol. 17, Issue 10, pp. 5771-83, 1997 (PubMed).

Knudsen, Wang: "Differential regulation of retinoblastoma protein function by specific Cdk phosphorylation sites." in: The Journal of biological chemistry, Vol. 271, Issue 14, pp. 8313-20, 1996 (PubMed).

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