Eukaryotic Translation Initiation Factor 2-alpha Kinase 2 (EIF2AK2) (pThr451) antibody

Details for Product No. ABIN197058
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Synonyms PKR, pkr, EIF2AK2, DKFZp469K1934, EIF2AK1, PRKR, 2310047A08Rik, 4732414G15Rik, AI467567, AI747578, Pkr, Prkr, Tik
(53), (49), (21), (15), (12), (12), (10), (8), (7), (6), (4), (4), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
(243), (57), (28)
(231), (18)
(5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (1), (1), (1)
Immunohistochemistry (Frozen Paraffin-embedded Sections) (IHC (frpe)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(158), (110), (93), (52), (40), (39), (16), (13), (5), (4), (2)
Pubmed 3 references available
Quantity 0.1 mL
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Catalog No. ABIN197058
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Immunogen The antiserum was produced against synthesized phosphopeptide derived from human PKR around the phosphorylation site of threonine 451 (K-G-TP-L-R).
Specificity PKR antibody detects endogenous levels of PKR only when phosphorylated at threonine 451.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Alternative Name EIF2AK2 / PKR
Background PKR is an interferon-inducible serine/threonine specific protein kinase. It is widely expressed in eukaryotic organisms and activated by double stranded RNA. Activation of PKR by dsRNAs leads to autophosphorylation at multiple sites. Phosphorylation of Thr446 and Thr451 in the PKR activation loop is required in vivo and in vitro for high level kinase activity. PKR phosphorylates its natural substrate, the alpha subunit of eukaryotic protein synthesis initiation factor 2 (EIF2 alpha), leading to the inhibition of protein synthesis. PKR is also involved in TLR signaling and mediates apoptosis in fibroblasts in response to viral infection and inflammatory cytokines, and also activates IKK and NFKB, thereby suppressing apoptosis. Recently, it has been reported that PKR also phosphorylates human p53 on serine 392. PKR might play a role in ER stress-induced apoptosis and in Alzheimer's disease. Alzheimer cases show prominent PKR activation in association with neuritic plaques and pyramidal neurons in the hippocampus and neocortex.Synonyms: Eukaryotic translation initiation factor 2-alpha kinase 2, Interferon-induced double-stranded RNA-activated protein kinase, PRKR, Protein kinase RNA-activated, eIF-2A protein kinase 2, p68 kinase
Gene ID 5610
NCBI Accession NP_001129123
UniProt P19525
Application Notes Immunohistochemistry: 1: 50approx. 1: 100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer PBS(without Mg2+ and Ca2+), pH 7.4 containing 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Supplier Images
anti-Eukaryotic Translation Initiation Factor 2-alpha Kinase 2 (EIF2AK2) (pThr451) antibody anti-Eukaryotic Translation Initiation Factor 2-alpha Kinase 2 (EIF2AK2) (pThr451) antibody
Background publications Hebner, Wilson, Rader et al.: "Human papillomaviruses target the double-stranded RNA protein kinase pathway." in: The Journal of general virology, Vol. 87, Issue Pt 11, pp. 3183-93, 2006 (PubMed).

Ruf, Lackey, Warudkar et al.: "Protection from interferon-induced apoptosis by Epstein-Barr virus small RNAs is not mediated by inhibition of PKR." in: Journal of virology, Vol. 79, Issue 23, pp. 14562-9, 2005 (PubMed).

van Huizen, Martindale, Gorospe et al.: "P58IPK, a novel endoplasmic reticulum stress-inducible protein and potential negative regulator of eIF2alpha signaling." in: The Journal of biological chemistry, Vol. 278, Issue 18, pp. 15558-64, 2003 (PubMed).

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