HLAG antibody

Catalog No. ABIN206902

This Mouse Monoclonal antibody specifically detects HLAG in ELISA, FACS, IHC, IP, IHC (fro) and ICC. It exhibits reactivity toward Human.

Quick Overview for HLAG antibody (ABIN206902)

Target: HLAG (HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This HLAG antibody is un-conjugated

Application: ELISA, Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC)

Clone: MEM-G-9

Product Details anti-HLAG Antibody

Specificity: Reacts with native form of human HLA-G1 on the cell surface as well as with soluble HLA-G5 isoform in its beta2-microglobulin associated form

Purification: Precipitation

Immunogen: Recombinant human HLA-G refolded with beta2-microglobulin and peptide.

Type of Immunogen: Recombinant protein

Isotype: IgG1

Application Details

Application Notes: Approved: ELISA (2 - 5 μg/mL), Flo (2 - 5 μg/mL), ICC, IHC, IHC-Fr (2 - 5 μg/mL), IP (2 - 5 μg/mL)

Usage: Flow Cytometry: Recommended dilution - 1-5 μg/mL. Positive control - JEG-3 human choriocarcinoma cell line. Immunocytochemistry: Recommended dilution -. Alexa Fluor®. 488 Fab-fragment: 5 μg/mL. ELISA: Application note - The antibody MEM-G/9 has been t The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.

Not recommended for: IHC-P

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Azide free PBS, 0.3 M sodium chloride, preserved by filter sterilization, approx,  pH 7.4.

Preservative: Azide free

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for HLAG

Target: HLAG (HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG))

Alternative Name: HLA-G

Background: Name/Gene ID: HLA-G

Synonyms: HLA-G, HLAG, HLA-6.0, MHC class I antigen G, HLA G antigen

Gene ID: 3135

UniProt: P17693

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints, Human Leukocyte Antigen (HLA) in Adaptive Immune Response