STAT1 antibody (C-Term)

Catalog No. ABIN212695

This Rabbit Polyclonal antibody specifically detects STAT1 in WB and IP. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for STAT1 antibody (C-Term) (ABIN212695)

Target: STAT1 (Signal Transducer and Activator of Transcription 1, 91kDa (STAT1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This STAT1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Product Details anti-STAT1 Antibody

Binding Specificity: C-Term

Specificity: Recognizes the STAT1 protein, Mr 91kD. of human, mouse and rat. Other species cross-reactivity is unknown.

Purification: Protein A purified

Immunogen: GST fusion-protein containing the carboxy terminal 36 amino acids of human STAT1a. This sequence is missing in the b isoform.

Type of Immunogen: GST fusion protein

Isotype: IgG

Application Details

Application Notes: Approved: IP, WB (0.5 - 2 μg/mL)

Usage: Suitable for use in Western Blot, Immunoprecipitation. Western Blot: 0.5-2 μg/mL detects STAT1 in RIPA lysates from human Jurkat, mouse CTLL and rat L6 cells. Jurkat cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-STAT1 (1 μg/mL). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunoprecipitation: 4 mg immunoprecipitated STAT1 from 500 mg of human Jurkat RIPA lysate.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 250 μLof 0.07 M Tris-glycine, 0.105 M sodium chloride, 0.035 % sodium azide, 30 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for STAT1

Target: STAT1 (Signal Transducer and Activator of Transcription 1, 91kDa (STAT1))

Alternative Name: STAT1

Background: Name/Gene ID: STAT1

Synonyms: STAT1, ISGF-3, STAT91, CANDF7

Gene ID: 6772

UniProt: P42224

Pathways: JAK-STAT Signaling, RTK Signaling, Interferon-gamma Pathway, Response to Growth Hormone Stimulus, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Endopeptidase Activity, Hepatitis C, CXCR4-mediated Signaling Events