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Lipopolysaccharide-Induced Tumor Necrosis Factor-alpha Factor (LITAF) (Internal Region) antibody

Details for Product No. ABIN238579, Supplier: Login to see
Antigen
  • PIG7
  • SIMPLE
  • TP53I7
  • EET-1
  • Pig7
  • TNF-alpha
  • 3222402J11Rik
  • C85531
  • N4WBP3
  • TBX1
Epitope
Internal Region
17
7
6
4
2
2
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
59
10
10
1
1
1
1
1
Host
Goat
24
19
17
Clonality
Polyclonal
Conjugate
Un-conjugated
1
1
1
1
1
1
Methode Type
Peptide ELISA
Application
ELISA, Western Blotting (WB)
35
31
9
8
8
5
1
1
1
1
Supplier
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Immunogen Peptide with sequence C-PDGKGMNPPSYYTQ, from the internal region of the protein sequence according to NP_004853.2, NP_001129945.1.
Blocking Peptide Blocking peptide for this product available: ABIN369534
Specificity This antibody is excpected to crossreact with both reported isoforms (NP_004853.2, NP_001129945.1).
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name LITAF (LITAF Antibody Abstract)
Background Synonyms: LITAF, lipopolysaccharide-induced TNF factor, CMT1C, FLJ38636, MGC116698, MGC116700, MGC116701, MGC125274, MGC125275, MGC125276, PIG7, SIMPLE, TP53I7, LPS-induced TNF-alpha factor, small integral membrane protein of lysosome/late endosome, tumor protein
Gene ID 9516, 56722, 65161
NCBI Accession NP_001129945, NP_004853
Research Area Chromatin and Nuclear Signaling, Transcription Factors
Pathways
Application Notes ELISA: antibody detection limit dilution 1:8000.
Western blot: Preliminary experiments gave an approx 25 kDa band in Human Lymph Node lysates and in lysates of cell lines HeLa and HepG2 after 0.1 µg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 17.1 kDa according to NP_004853.2. The 25 kDa band was successfully blocked by incubation with the immunizing peptide.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Background publications Tang, Metzger, Leeman et al.: "LPS-induced TNF-alpha factor (LITAF)-deficient mice express reduced LPS-induced cytokine: Evidence for LITAF-dependent LPS signaling pathways." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 37, pp. 13777-82, 2006 (PubMed).