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Lipopolysaccharide-Induced Tumor Necrosis Factor-alpha Factor (LITAF) (Internal Region) antibody

Details for Product No. ABIN238579
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Antigen
Synonyms PIG7, SIMPLE, TP53I7, EET-1, Pig7, TNF-alpha
Epitope
Internal Region
(9), (2), (2), (1)
Reactivity
Human, Mouse (Murine), Rat (Rattus)
(31), (4), (4)
Host
Goat
(18), (10), (4)
Clonality
Polyclonal
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB)
(19), (16), (4), (3), (2), (1), (1)
Pubmed 1 reference available
Quantity 100 µg
Options
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Catalog No. ABIN238579
261.61 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen Peptide with sequence C-PDGKGMNPPSYYTQ, from the internal region of the protein sequence according to NP_004853.2, NP_001129945.1.
Specificity This antibody is excpected to crossreact with both reported isoforms (NP_004853.2, NP_001129945.1).
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name LITAF
Background Synonyms: LITAF, lipopolysaccharide-induced TNF factor, CMT1C, FLJ38636, MGC116698, MGC116700, MGC116701, MGC125274, MGC125275, MGC125276, PIG7, SIMPLE, TP53I7, LPS-induced TNF-alpha factor, small integral membrane protein of lysosome/late endosome, tumor protein
Gene ID 9516, 56722, 65161
NCBI Accession NP_004853.2, NP_001129945.1
Research Area Chromatin and Nuclear Signaling, Transcription Factors
Application Notes ELISA: antibody detection limit dilution 1:8000.
Western blot: Preliminary experiments gave an approx 25 kDa band in Human Lymph Node lysates and in lysates of cell lines HeLa and HepG2 after 0.1 µg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 17.1 kDa according to NP_004853.2. The 25 kDa band was successfully blocked by incubation with the immunizing peptide.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
General Tang, Metzger, Leeman et al.: "LPS-induced TNF-alpha factor (LITAF)-deficient mice express reduced LPS-induced cytokine: Evidence for LITAF-dependent LPS signaling pathways." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 37, pp. 13777-82, 2006 (PubMed).

Hosts (18), (10), (4)
Reactivities (31), (4), (4)
Applications (19), (16), (4), (3), (2), (1), (1)
Epitopes (9), (2), (2), (1)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN238579
261.61 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
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