Perforin 1 antibody (Perforin 1 (Pore Forming Protein)) (Alexa Fluor 647)

Details for Product anti-PRF1 Antibody No. ABIN2657991, Supplier: Log in to see
Antigen
  • PRF1
  • LOC443187
  • perforin
  • prf1
  • FLH2
  • HPLH2
  • P1
  • PFN1
  • PFP
  • Pfn
  • Pfp
  • Prf-1
  • Cyta
  • RATCYTA
  • perforin 1 (pore forming protein)
  • perforin
  • perforin 1-like
  • PRF1
  • LOC443187
  • prf1
  • Prf1
  • LOC100342396
Alternatives
anti-Human Perforin 1 antibody for Immunohistochemistry (Frozen Sections)
Reactivity
Human
158
46
24
22
2
2
1
1
1
Host
Mouse
100
75
20
Clonality (Clone)
Monoclonal ()
Conjugate
This Perforin 1 antibody is conjugated to Alexa Fluor 647
16
12
10
5
3
3
3
3
2
2
2
2
2
2
2
2
2
2
Application
Flow Cytometry (FACS)
98
81
46
41
38
37
35
34
31
6
6
6
3
2
2
1
1
1
1
1
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Clone DG9
Isotype IgG2b kappa
Cross-Reactivity Cow (Bovine)
No Cross-Reactivity Mouse (Murine)
Purification The antibody was purified by affinity chromatography, and conjugated with Alexa Fluor® 647 under optimal conditions.
Alternative Name Perforin (PRF1 Antibody Abstract)
Background Perforin is a 70 kD cytolytic protein that is expressed in the cytoplasmic granules of cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells. Perforin is one of the major effector molecules used by cytotoxic T cells and NK cells to mediate targeted cell lysis.
Research Area Immunology, Innate Immunity, Adaptive Immunity, Inflammation
Pathways Apoptosis, Caspase Cascade in Apoptosis
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Buffer Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide and 0.2 % (w/v) BSA .
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Protect from prolonged exposure to light. Do not freeze.
Storage 4 °C
Storage Comment The antibody solution should be stored undiluted between 2°C and 8°C
Supplier Images
Flow Cytometry (FACS) image for anti-Perforin 1 (Pore Forming Protein) (PRF1) antibody (Alexa Fluor 647) (ABIN2657991) anti-Perforin 1 (Pore Forming Protein) (PRF1) antibody (Alexa Fluor 647)
Background publications Pérez-Quintero, Roncagalli, Guo, Latour, Davidson, Veillette: "EAT-2, a SAP-like adaptor, controls NK cell activation through phospholipase Cγ, Ca++, and Erk, leading to granule polarization." in: The Journal of experimental medicine, Vol. 211, Issue 4, pp. 727-42, 2014 (PubMed).

Makedonas, Hutnick, Haney, Amick, Gardner, Cosma, Hersperger, Dolfi, Wherry, Ferrari, Betts: "Perforin and IL-2 upregulation define qualitative differences among highly functional virus-specific human CD8 T cells." in: PLoS pathogens, Vol. 6, Issue 3, pp. e1000798, 2010 (PubMed).

Wood, Meeths, Chiang, Bechensteen, Boelens, Heilmann, Horiuchi, Rosthøj, Rutynowska, Winiarski, Stow, Nordenskjöld, Henter, Ljunggren, Bryceson: "Different NK cell-activating receptors preferentially recruit Rab27a or Munc13-4 to perforin-containing granules for cytotoxicity." in: Blood, Vol. 114, Issue 19, pp. 4117-27, 2009 (PubMed).

Chen, Hou, Jiang, Ma, Meng, Wang, Zhang, Zhang, Tang, Zhang, Wan, Li, Yu, Hu, Yang, He, Wang, Cao: "Response of memory CD8+ T cells to severe acute respiratory syndrome (SARS) coronavirus in recovered SARS patients and healthy individuals." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 175, Issue 1, pp. 591-8, 2005 (PubMed).

Mauad, van Schadewijk, Schrumpf, Hack, Fernezlian, Garippo, Ejzenberg, Hiemstra, Rabe, Dolhnikoff: "Lymphocytic inflammation in childhood bronchiolitis obliterans." in: Pediatric pulmonology, Vol. 38, Issue 3, pp. 233-9, 2004 (PubMed).

Schaerli, Ebert, Willimann, Blaser, Roos, Loetscher, Moser: "A skin-selective homing mechanism for human immune surveillance T cells." in: The Journal of experimental medicine, Vol. 199, Issue 9, pp. 1265-75, 2004 (PubMed).

Lieberman: "The ABCs of granule-mediated cytotoxicity: new weapons in the arsenal." in: Nature reviews. Immunology, Vol. 3, Issue 5, pp. 361-70, 2003 (PubMed).

Trapani, Smyth: "Functional significance of the perforin/granzyme cell death pathway." in: Nature reviews. Immunology, Vol. 2, Issue 10, pp. 735-47, 2002 (PubMed).

Barrat, Le Deist, Benkerrou, Bousso, Feldmann, Fischer, de Saint Basile: "Defective CTLA-4 cycling pathway in Chediak-Higashi syndrome: a possible mechanism for deregulation of T lymphocyte activation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, Issue 15, pp. 8645-50, 1999 (PubMed).

Watanabe, De Rosa, Cmelak, Hoppe, Herzenberg, Roederer: "Long-term depletion of naive T cells in patients treated for Hodgkin's disease." in: Blood, Vol. 90, Issue 9, pp. 3662-72, 1997 (PubMed).

Hameed, Olsen, Cheng, Fox, Hruban, Podack: "Immunohistochemical identification of cytotoxic lymphocytes using human perforin monoclonal antibody." in: The American journal of pathology, Vol. 140, Issue 5, pp. 1025-30, 1992 (PubMed).

Did you look for something else?