X-ray Repair, Complementing Defective, Repair in Chinese Hamster (XRCC5) antibody

Antigen: X-ray Repair, Complementing Defective, Repair in Chinese Hamster (XRCC5)

Synonyms: KU80, KUB2, Ku86, NFIV, KARP1, KARP-1, FLJ39089

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Catalog no.: ABIN271897

Additional Information

Characteristics: Synonyms: TLAA, G22P2, CTCBF, CTC85, DNA repair protein XRCC5, ATP-dependent DNA helicase II 80kDa subunit, Lupus Ku autoantigen protein p86, 86 kDa subunit of Ku antigen,Thyroid-lupus autoantigen, CTC box-binding factor 85 kDa subunit, Nuclear factor IV

Alternative name: XRCC5 / Ku80 / Ku86

Swiss-Prot: P13010

Cross-Reactivity: Human, Mouse (Murine)

Description: The Ku protein is localized in the nucleus and is composed of subunits referred to as Ku-70(or p70) and Ku-86 (or p86), which is also known by the synonym Ku-80 (or p80). Ku wasfirst described as an autoantigen to which antibodies were produced in a patient withscleroderma-polymyositis overlap syndrome, and was later found in the sera of patientswith other rheumatic diseases. Both subunits of the Ku protein have been cloned, and anumber of functions have been proposed for Ku, including cell signaling, DNA replicationand transcriptional activation. Ku is involved in Pol II-directed transcription by virtue of itsDNA-binding activity, serving as the regulatory component of the DNA-associated proteinkinase that phosphorylates Pol II and transcription factor Sp. Ku proteins also activatetranscription from the U1 small nuclear RNA and the human transferrin receptor genepromoters. A Ku-related protein designated the enhancer 1 binding factor (E1BF),composed of two subunits, has been identified as a positive regulator of RNA polymerase Itranscription initiation.

Specificity: Ku80 (D708) antibody detects endogenous levels of Ku80 protein. Species: Human, Mouse. Others not tested.

Application Details

Application Notes: ELISA: 1: 5000~1: 10000. WB: 1: 500~1: 1000. IHC: 1: 50~1: 200. IF: 1: 50~1: 200. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 1,0 mg/ml

Purification: Aff - Purified

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.2.

Storage: Store the antibody undiluted at 2-8°C. DO NOT FREEZE! Shelf life: one year from despatch.

Restrictions: For Research Use only