XRCC5 antibody (C-Term)

Catalog No. ABIN2778271

This Rabbit Polyclonal antibody specifically detects XRCC5 in WB and IHC. It exhibits reactivity toward Human, Mouse, Rat, Dog, Horse, Cow, Guinea Pig and Rabbit.

Quick Overview for XRCC5 antibody (C-Term) (ABIN2778271)

Target: XRCC5 (X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 5 (Double-Strand-Break Rejoining) (XRCC5))

Reactivity: Human, Mouse, Rat, Dog, Horse, Cow, Guinea Pig, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This XRCC5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-XRCC5 Antibody

Binding Specificity: C-Term

Sequence: GITLITKEEA SGSSVTAEEA KKFLAPKDKP SGDTAAVFEE GGDVDDLLDM

Predicted Reactivity: Cow: 92%, Dog: 92%, Guinea Pig: 93%, Horse: 100%, Human: 100%, Mouse: 85%, Rabbit: 93%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against XRCC5. It was validated on Western Blot and immunohistochemistry.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human XRCC5

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 732 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for XRCC5

Target: XRCC5 (X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 5 (Double-Strand-Break Rejoining) (XRCC5))

Alternative Name: XRCC5

Background: XRCC5 encodes the 80-kilodalton subunit of the Ku heterodimer protein which is also known as ATP-dependant DNA helicase II or DNA repair protein XRCC5. Ku is the DNA-binding component of the DNA-dependent protein kinase, and it functions together with the DNA ligase IV-XRCC4 complex in the repair of DNA double-strand break by non-homologous end joining and the completion of V(D)J recombination events. This gene functionally complements Chinese hamster xrs-6, a mutant defective in DNA double-strand break repair and in ability to undergo V(D)J recombination. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity.
Alias Symbols: KU80, KUB2, Ku86, NFIV, KARP1, KARP-1
Protein Interaction Partner: HUWE1, UBC, EHF, ELF3, SUMO2, SUMO3, BRCA1, ATRX, Fbxl16, NEDD8, SUMO1, MDM2, RPA3, RPA2, RPA1, SUZ12, EED, RNF2, BMI1, EIF4B, C1QBP, RPL24, PLCB3, PRMT3, EIF3J, CUL2, CSDE1, SF1, TRMT1, SAMHD1, DNMBP, GANAB, HYOU1, NCL, NARS, MSH2, JUP, HSPA4, PARP1, TAR
Protein Size: 732

Molecular Weight: 83 kDa

Gene ID: 7520

NCBI Accession: NM_021141, NP_066964

UniProt: Q4VBQ5

Pathways: DNA Damage Repair