XRCC5 antibody (AA 251-455)

Catalog No. ABIN7175897

Product Details anti-XRCC5 Antibody

Target: XRCC5 (X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 5 (Double-Strand-Break Rejoining) (XRCC5))

Binding Specificity: AA 251-455

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This XRCC5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP)

Cross-Reactivity: Human

Purification: >95%, Protein G purified

Immunogen: Recombinant Human X-ray repair cross-complementing protein 5 protein (251-455AA)

Isotype: IgG

Application Details

Application Notes: Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IP:1:200-1:2000,

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, pH 7.4

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for XRCC5

Target: XRCC5 (X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 5 (Double-Strand-Break Rejoining) (XRCC5))

Alternative Name: XRCC5 (XRCC5 Products)

Synonyms: AI314015 antibody, Ku80 antibody, Ku86 antibody, Kup80 antibody, KARP-1 antibody, KARP1 antibody, KU80 antibody, KUB2 antibody, NFIV antibody, xrcc5-a antibody, XRCC5 antibody, X-ray repair cross complementing 5 antibody, X-ray repair complementing defective repair in Chinese hamster cells 5 antibody, X-ray repair complementing defective repair in Chinese hamster cells 5 L homeolog antibody, XRCC5 antibody, Xrcc5 antibody, xrcc5.L antibody

Background:

Background: Single-stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3\\\'-5\\\' direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The XRCC5/6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5/6 dimer is probably involved in stabilizing broken DNA ends and bringing them together (PubMed:12145306, PubMed:20383123, PubMed:7957065, PubMed:8621488). The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. In association with NAA15, the XRCC5/6 dimer binds to the osteocalcin promoter and activates osteocalcin expression (PubMed:20383123). The XRCC5/6 dimer probably also acts as a 5\\\'-deoxyribose-5-phosphate lyase (5\\\'-dRP lyase), by catalyzing the beta-elimination of the 5\\\' deoxyribose-5-phosphate at an abasic site near double-strand breaks. XRCC5 probably acts as the catalytic subunit of 5\\\'-dRP activity, and allows to \\\'clean\\\' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5/6 dimer together with APEX1 acts as a negative regulator of transcription (PubMed:8621488).

Aliases: 86 kDa subunit of Ku antigen antibody, ATP dependent DNA helicase 2 subunit 2 antibody, ATP dependent DNA helicase II 80 kDa subunit antibody, ATP dependent DNA helicase II 86 Kd subunit antibody, ATP dependent DNA helicase II antibody, ATP-dependent DNA helicase 2 subunit 2 antibody, ATP-dependent DNA helicase II 80 kDa subunit antibody, CTC box binding factor 85 kDa antibody, CTC box-binding factor 85 kDa subunit antibody, CTC85 antibody, CTCBF antibody, DNA repair protein XRCC5 antibody, KARP 1 antibody, KARP1 antibody, Ku 80 antibody, Ku autoantigen 80 kDa antibody, Ku80 antibody, Ku86 antibody, Ku86 autoantigen related protein 1 antibody, KUB 2 antibody, KUB2 antibody, Lupus Ku autoantigen protein p86 antibody, NFIV antibody, Nuclear factor IV antibody, Thyroid lupus autoantigen antibody, Thyroid-lupus autoantigen antibody, TLAA antibody, X ray repair complementing defective repair in Chinese hamster cells 5 (double strand break rejoining) antibody, X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining) antibody, X-ray repair cross-complementing protein 5 antibody, Xray repair complementing defective repair in Chinese hamster cells 5 antibody, XRCC 5 antibody, XRCC5 antibody, XRCC5_HUMAN antibody

UniProt: P13010

Pathways: DNA Damage Repair