COPS5
Reactivity: Human
WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro)
Host: Rabbit
Polyclonal
unconjugated
Application Notes
Immunoprecipitation. Western blot. Immunohistochemistry on paraffin sections (5 μg/mL). Antigen retrieval: Steam slides in 0.01 M sodium citrate buffer, pH 6.0, at 99-100C for 20 min. Remove fromheat and let stand at room temperature in buffer for 20 min. Rinse in 1x TBS with Tween(TBST) for 1 min. at room temperature. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Buffer
Phosphate buffered saline
Storage
-20 °C
Storage Comment
Store the antibody (in aliquots) at -20 °C to -70 °C. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
JAB-1 is a 38 kDa protein which was originally described as a coactivator for cJUN. It is present in a large protein complex called the "Signalosome", which has been proposed to play a signaling role in mammalian cells. This protein complex appears to be analogous to the COP9 complex in plants, which is important in light-mediated plant development. Although the specific function of JAB-1 in this complex is unclear, it has recently been implicated in targeting proteins for degradation by the proteasome.Synonyms: COP9 signalosome complex subunit 5, CSN5, JAB1, Jun activation domain-binding protein 1, SGN5, Signalosome subunit 5