TNFRSF10B antibody (C-Term)

Catalog No. ABIN3044205

Product Details anti-TNFRSF10B Antibody

Target: TNFRSF10B (Tumor Necrosis Factor Receptor Superfamily, Member 10b (TNFRSF10B))

Binding Specificity: AA 333-348, C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TNFRSF10B antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Tumor necrosis factor receptor superfamily member 10B(TNFRSF10B) detection. Tested with WB, IHC-P in Human.

Sequence: DPTETLRQCF DDFADL

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Tumor necrosis factor receptor superfamily member 10B(TNFRSF10B) detection. Tested with WB, IHC-P in Human.
Gene Name: tumor necrosis factor receptor superfamily, member 10b
Protein Name: Tumor necrosis factor receptor superfamily member 10B

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human DR5(333-348aa DPTETLRQCFDDFADL).

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Expiry Date: 12 months

References for anti-TNFRSF10B antibody (ABIN3044205)

Liu, Zuo, Wang, Chen, Yang, Wang, Zhu: "miR-942 decreases TRAIL-induced apoptosis through ISG12a downregulation and is regulated by AKT." in: Oncotarget, Vol. 5, Issue 13, pp. 4959-71, (2014) (PubMed).

Li, Shen, Peng, Chen, Jiang, Li, Zhang, Lu: "Plumbagin enhances TRAIL-mediated apoptosis through up-regulation of death receptor in human melanoma A375 cells." in: Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban, Vol. 30, Issue 4, pp. 458-63, (2010) (PubMed).

Target Details for TNFRSF10B

Target: TNFRSF10B (Tumor Necrosis Factor Receptor Superfamily, Member 10b (TNFRSF10B))

Alternative Name: TNFRSF10B (TNFRSF10B Products)

Synonyms: TNFRSF10B antibody, dr5 antibody, cd262 antibody, killer antibody, trick2 antibody, trickb antibody, xDR-M1 antibody, xDR-M2 antibody, ztnfr9 antibody, trailr2 antibody, trick2a antibody, trick2b antibody, CD262 antibody, DR5 antibody, KILLER antibody, KILLER/DR5 antibody, TRAIL-R2 antibody, TRAILR2 antibody, TRICK2 antibody, TRICK2A antibody, TRICK2B antibody, TRICKB antibody, ZTNFR9 antibody, Ly98 antibody, MK antibody, tumor necrosis factor receptor superfamily, member 10b antibody, tumor necrosis factor receptor superfamily member 10b S homeolog antibody, tumor necrosis factor receptor superfamily member 10b L homeolog antibody, tumor necrosis factor receptor superfamily member 10b antibody, TNF receptor superfamily member 10b antibody, Tnfrsf10b antibody, TNFRSF10B antibody, tnfrsf10b.S antibody, tnfrsf10b.L antibody, tnfrsf10b antibody

Background: TNFRSF10B(Tumor necrosis factor receptor superfamily, member 10b) is a human gene. It is also known as DR5, CD262, KILLER, TRICK2, TRICKB, ZTNFR9, TRAILR2, TRICK2A, TRICK2B, TRAIL-R2, KILLER/DR5. The protein encoded by this gene is a member of the TNF-receptor superfamily, and contains an intracellular death domain. This receptor can be activated by tumor necrosis factor-related apoptosis inducing ligand(TNFSF10/TRAIL/APO-2L), and transduces apoptosis signal. Mice have a homologous gene, tnfrsf10b, that has been essential in the elucidation of the function of this gene in humans. Studies with FADD-deficient mice suggested that FADD, a death domain containing adaptor protein, is required for the apoptosis mediated by this protein.By analysis of radiation hybrid panels, Walczak et al.(1997) mapped the TRAILR2 gene to chromosome 8p22-p21. Northern blot analysis indicated that TRAILR2 was expressed as a 4.4-kb mRNA in all tissues tested, with the highest levels of expression in peripheral blood lymphocytes, spleen, and ovary.

Synonyms: Fas like protein antibody|Apoptosis inducing protein TRICK2A/2B antibody|Apoptosis inducing receptor TRAIL R2 antibody|CD262 antibody|CD262 antigen antibody|Cytotoxic TRAIL receptor 2 antibody|Death domain containing receptor for TRAIL/Apo 2L antibody|Death receptor 5 antibody|DR5 antibody|KILLER antibody|KILLER/DR5 antibody|OTTHUMP00000123492 antibody|OTTHUMP00000123493 antibody|p53 regulated DNA damage inducible cell death receptor(killer) antibody|TNF related apoptosis inducing ligand receptor 2 antibody|TNF-related apoptosis-inducing ligand receptor 2 antibody|TNFRSF10B antibody|TR10B_HUMAN antibody|TRAIL R2 antibody|TRAIL receptor 2 antibody|TRAIL-R2 antibody|TRAILR2 antibody|TRICK2 antibody|TRICK2A antibody|TRICK2B antibody|TRICKB antibody|Tumor necrosis factor receptor like protein ZTNFR9 antibody|Tumor necrosis factor receptor superfamily member 10B antibody|Tumor necrosis factor receptor superfamily, member 10b antibody|ZTNFR9 antibody

UniProt: O14763

Pathways: p53 Signaling, Apoptosis, Positive Regulation of Endopeptidase Activity