AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (pThr450)

Details for Product anti-AKT1 Antibody No. ABIN319271, Supplier: Log in to see
Antigen
  • AKT
  • AKT1
  • pkb
  • xAct
  • v-akt
  • v-akt1
  • akt-1
  • AKT/PKB
  • Akt
  • Akt/PKB
  • Akt1
  • PKB
  • CG4006
  • D-Akt
  • DAKT1
  • DAKT1/PKB
  • DAkt
  • DAkt1
  • DPKB
  • DRAC-PK
  • DRAC-PK66
  • DRAC-PK85
  • Dakt
  • Dakt1
  • Dmel\\CG4006
  • Dpkb
  • PKB/AKT
  • PKB/Akt
  • PKB/dAKT
  • RAC
  • RacPK
  • akt
  • akt1
  • dAKT
  • dAKT/dPKB
  • dAKT1
  • dAkt
  • dAkt/PKB
  • dAkt1
  • dPKB
  • dakt
  • dakt1
  • l(3)04226
  • l(3)89Bq
  • p-Akt
  • pAkt
  • ATAKT1
  • F18A8.2
  • F18A8_2
  • K+ transporter 1
  • POTASSIUM TRANSPORTER
  • Rac
  • PKBalpha
  • CWS6
  • PKB-ALPHA
  • PRKBA
  • RAC-ALPHA
  • v-akt murine thymoma viral oncogene homolog 1
  • CG4006 gene product from transcript CG4006-RA
  • potassium channel AKT1
  • thymoma viral proto-oncogene 1
  • AKT1
  • akt1
  • Akt1
  • KT1
Alternatives
anti-Human AKT antibody for Western Blotting
Epitope
pThr450
222
130
115
66
56
51
40
37
22
22
22
21
18
16
13
12
12
12
11
11
10
10
9
9
9
8
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7
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6
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4
4
4
4
4
4
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
1262
654
508
63
58
56
46
35
33
30
18
8
8
6
5
5
3
3
3
2
1
1
1
1
1
Host
Rabbit
922
384
12
4
2
Clonality
Polyclonal
Conjugate
This AKT antibody is un-conjugated
54
42
40
38
28
25
15
15
10
10
8
6
6
6
6
6
6
6
5
5
5
4
4
2
2
2
2
2
2
2
2
1
1
1
1
Application
Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
1076
562
482
258
188
174
128
84
67
33
26
15
13
11
11
4
4
3
3
3
2
1
1
1
1
1
1
1
Options
Supplier
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Supplier Product No.
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Immunogen The antiserum was produced against synthesized phosphopeptide derived from human AKT1 around the phosphorylation site of threonine 450 (T-I-TP-P-P).
Specificity AKT1 (phospho-Thr450) Antibody AP08031PU detects endogenous levels of AKT1 only when phosphorylated at Threonine 450.
Purification Immunoaffinity Chromatography: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Alternative Name AKT1 / PKB (AKT1 Antibody Abstract)
Background Synonyms: Akt-1, C-AKT, Protein kinase B, RAC-PK-alpha, RAC-alpha serine/threonine-protein kinase
Gene ID 207
NCBI Accession NP_001014431
UniProt P31749
Research Area Signaling, Protein Modifications, Kinases/Phosphatases
Pathways PI3K-Akt Signaling, RTK Signaling, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Response to Water Deprivation, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, Thromboxane A2 Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, Positive Regulation of fat Cell Differentiation, VEGFR1 Specific Signals, VEGF Signaling
Application Notes Immunohistochemistry (1/50-1/100). Immunofluorescence (1/100-1/200).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Buffer PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store the antibody (in aliquots) at-20 °C.
Supplier Images
 image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (pThr450) antibody (ABIN319271) anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (pThr450) antibody
 image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (pThr450) antibody (ABIN319271) anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (pThr450) antibody (Image 2)
Background publications Sarbassov, Guertin, Ali, Sabatini: "Phosphorylation and regulation of Akt/PKB by the rictor-mTOR complex." in: Science (New York, N.Y.), Vol. 307, Issue 5712, pp. 1098-101, 2005 (PubMed).

Salvioli, Bonafè, Capri, Monti, Franceschi: "Mitochondria, aging and longevity--a new perspective." in: FEBS letters, Vol. 492, Issue 1-2, pp. 9-13, 2001 (PubMed).

Gesbert, Sellers, Signoretti, Loda, Griffin: "BCR/ABL regulates expression of the cyclin-dependent kinase inhibitor p27Kip1 through the phosphatidylinositol 3-Kinase/AKT pathway." in: The Journal of biological chemistry, Vol. 275, Issue 50, pp. 39223-30, 2001 (PubMed).

Cantley, Neel: "New insights into tumor suppression: PTEN suppresses tumor formation by restraining the phosphoinositide 3-kinase/AKT pathway." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, Issue 8, pp. 4240-5, 1999 (PubMed).

Levin: "It's prime time for reverse transcriptase." in: Cell, Vol. 88, Issue 1, pp. 5-8, 1997 (PubMed).

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