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AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (pThr450)

Details for Product anti-AKT1 Antibody No. ABIN319271, Supplier: Log in to see
Antigen
  • AKT
  • AKT1
  • pkb
  • xAct
  • v-akt
  • v-akt1
  • akt-1
  • AKT/PKB
  • Akt
  • Akt/PKB
  • Akt1
  • PKB
  • CG4006
  • D-Akt
  • DAKT1
  • DAKT1/PKB
  • DAkt
  • DAkt1
  • DPKB
  • DRAC-PK
  • DRAC-PK66
  • DRAC-PK85
  • Dakt
  • Dakt1
  • Dmel\\CG4006
  • Dpkb
  • PKB/AKT
  • PKB/Akt
  • PKB/dAKT
  • RAC
  • RacPK
  • akt
  • akt1
  • dAKT
  • dAKT/dPKB
  • dAKT1
  • dAkt
  • dAkt/PKB
  • dAkt1
  • dPKB
  • dakt
  • dakt1
  • l(3)04226
  • l(3)89Bq
  • p-Akt
  • pAkt
  • ATAKT1
  • F18A8.2
  • F18A8_2
  • K+ transporter 1
  • POTASSIUM TRANSPORTER
  • Rac
  • PKBalpha
  • CWS6
  • PKB-ALPHA
  • PRKBA
  • RAC-ALPHA
  • v-akt murine thymoma viral oncogene homolog 1
  • CG4006 gene product from transcript CG4006-RA
  • potassium channel AKT1
  • thymoma viral proto-oncogene 1
  • AKT1
  • akt1
  • Akt1
  • KT1
Alternatives
anti-Human AKT antibody for Western Blotting
Epitope
pThr450
191
138
91
88
54
40
38
37
27
25
21
21
21
19
16
15
15
15
14
13
11
11
10
9
7
6
6
6
5
5
5
5
5
5
5
4
4
4
4
4
4
4
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
1186
548
412
44
42
37
34
32
20
18
11
6
5
4
2
2
2
1
1
Host
Rabbit
915
315
14
4
1
Clonality
Polyclonal
Conjugate
This AKT antibody is un-conjugated
46
39
35
34
26
25
10
10
5
5
5
4
4
4
3
3
3
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
Application
Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
984
559
477
224
143
133
120
71
63
36
24
11
11
5
3
3
3
3
2
1
1
1
1
1
1
1
Options
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Immunogen The antiserum was produced against synthesized phosphopeptide derived from human AKT1 around the phosphorylation site of threonine 450 (T-I-TP-P-P).
Specificity AKT1 (phospho-Thr450) Antibody AP08031PU detects endogenous levels of AKT1 only when phosphorylated at Threonine 450.
Purification Immunoaffinity Chromatography: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Alternative Name AKT1 / PKB (AKT1 Antibody Abstract)
Background Synonyms: Akt-1, C-AKT, Protein kinase B, RAC-PK-alpha, RAC-alpha serine/threonine-protein kinase
Gene ID 207
NCBI Accession NP_001014431
UniProt P31749
Research Area Signaling, Protein Modifications, Kinases/Phosphatases
Pathways PI3K-Akt Signaling, RTK Signaling, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway
Application Notes Immunohistochemistry (1/50-1/100). Immunofluorescence (1/100-1/200).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Buffer PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store the antibody (in aliquots) at-20 °C.
Supplier Images
 image for anti-AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (pThr450) (ABIN319271) anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (pThr450) antibody
 image for anti-AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (pThr450) (ABIN319271) anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (pThr450) antibody (Image 2)
Background publications Sarbassov, Guertin, Ali, Sabatini: "Phosphorylation and regulation of Akt/PKB by the rictor-mTOR complex." in: Science (New York, N.Y.), Vol. 307, Issue 5712, pp. 1098-101, 2005 (PubMed).

Salvioli, Bonafè, Capri, Monti, Franceschi: "Mitochondria, aging and longevity--a new perspective." in: FEBS letters, Vol. 492, Issue 1-2, pp. 9-13, 2001 (PubMed).

Gesbert, Sellers, Signoretti, Loda, Griffin: "BCR/ABL regulates expression of the cyclin-dependent kinase inhibitor p27Kip1 through the phosphatidylinositol 3-Kinase/AKT pathway." in: The Journal of biological chemistry, Vol. 275, Issue 50, pp. 39223-30, 2001 (PubMed).

Cantley, Neel: "New insights into tumor suppression: PTEN suppresses tumor formation by restraining the phosphoinositide 3-kinase/AKT pathway." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, Issue 8, pp. 4240-5, 1999 (PubMed).

Levin: "It's prime time for reverse transcriptase." in: Cell, Vol. 88, Issue 1, pp. 5-8, 1997 (PubMed).

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