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AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (pThr450)

Details for Product anti-AKT1 Antibody No. ABIN319272, Supplier: Log in to see
Antigen
Alternatives
anti-Human AKT antibody for Western Blotting
Epitope
pThr450
144
114
88
86
55
39
39
38
21
21
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Reactivity
Human, Mouse (Murine), Rat (Rattus)
1070
483
390
43
42
31
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20
17
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10
6
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4
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Host
Rabbit
882
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14
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1
Clonality
Polyclonal
Conjugate
This AKT antibody is un-conjugated
35
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10
5
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2
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1
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Application
Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
882
510
415
201
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107
107
65
43
36
33
12
11
6
3
3
3
3
1
1
1
1
1
Supplier
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Immunogen The antiserum was produced against synthesized phosphopeptide derived from human AKT1 around the phosphorylation site of threonine 450 (T-I-TP-P-P).
Specificity AKT1 (phospho-Thr450) Antibody AP08031PU detects endogenous levels of AKT1 only when phosphorylated at Threonine 450.
Purification Immunoaffinity Chromatography: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Alternative Name AKT1 / PKB (AKT1 Antibody Abstract)
Background Synonyms: Akt-1, C-AKT, Protein kinase B, RAC-PK-alpha, RAC-alpha serine/threonine-protein kinase
Gene ID 207
NCBI Accession NP_001014431
UniProt P31749
Research Area Signaling, Protein Modifications, Kinases/Phosphatases
Pathways PI3K-Akt Signaling, RTK Signaling, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway
Application Notes Immunohistochemistry (1/50-1/100). Immunofluorescence (1/100-1/200).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Buffer PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store the antibody (in aliquots) at-20 °C.
Supplier Images
 image for anti-AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (pThr450) (ABIN319272) anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (pThr450) antibody
 image for anti-AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (pThr450) (ABIN319272) anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (pThr450) antibody (Image 2)
Background publications Sarbassov, Guertin, Ali et al.: "Phosphorylation and regulation of Akt/PKB by the rictor-mTOR complex." in: Science (New York, N.Y.), Vol. 307, Issue 5712, pp. 1098-101, 2005 (PubMed).

Salvioli, Bonafè, Capri et al.: "Mitochondria, aging and longevity--a new perspective." in: FEBS letters, Vol. 492, Issue 1-2, pp. 9-13, 2001 (PubMed).

Gesbert, Sellers, Signoretti et al.: "BCR/ABL regulates expression of the cyclin-dependent kinase inhibitor p27Kip1 through the phosphatidylinositol 3-Kinase/AKT pathway." in: The Journal of biological chemistry, Vol. 275, Issue 50, pp. 39223-30, 2001 (PubMed).

Cantley, Neel: "New insights into tumor suppression: PTEN suppresses tumor formation by restraining the phosphoinositide 3-kinase/AKT pathway." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, Issue 8, pp. 4240-5, 1999 (PubMed).

Levin: "It's prime time for reverse transcriptase." in: Cell, Vol. 88, Issue 1, pp. 5-8, 1997 (PubMed).

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