Mast/stem Cell Growth Factor Receptor (KIT) antibody

Details for Product No. ABIN319615
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Antigen
Reactivity
Mouse (Murine)
(436), (323), (155), (105), (104), (91), (27), (5)
Host
Rat
(332), (157), (127), (2), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(45), (36), (36), (21), (19), (10), (10), (10), (10), (10), (10), (10), (10), (7), (6), (6), (4), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Flow Cytometry (FACS)
(258), (180), (115), (100), (89), (62), (34), (31), (25), (20), (10), (10), (7), (3), (2), (2)
Pubmed 6 references available
Catalog no. ABIN319615
Quantity 0.25 mg
Price
401.50 $   Plus shipping costs $45.00
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Immunogen IL-3 dependent mast cells. Spleen cells from immunised Wistar rats were fused with cells of the Fox-NY mouse myeloma cell line.
Clone 2B8
Isotype IgG2b
Specificity This antibody reacts with CD117 also known as Kit or c-kit receptor. Clone 2B8 recognises an epitope within the extracellular region of CD117.
Purification Affinity Chromatography on Protein G.
Alternative Name CD117 / c-kit
Background CD117 is the receptor of the steel factor (Stem Cell Factor). C-kit receptor is expressed on 5-10% of total adult bone marrow cells including multipotent hematopoietic cells.
Alternate names: KIT, Mast/stem cell growth factor receptor, Proto-Oncogene Tyrosine-Protein Kinase, SCFR
Gene ID 16590
NCBI Accession NP_066922.2
UniProt P05532
Research Area CD Antigens, Surface Receptors of Immune Cells, Growth Factors, Receptors, Proliferation Markers
Application Notes Flow Cytometry: Use 10 ul of 1/50-1/200 diluted antibody to label 10e6 cells. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS, pH 7.4 containing 0.09% Sodium Azide as preservative.
Preservative Sodium azide
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Expiry Date 12 months
Background publications Manova, Bachvarova, Huang et al.: "c-kit receptor and ligand expression in postnatal development of the mouse cerebellum suggests a function for c-kit in inhibitory interneurons." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 12, Issue 12, pp. 4663-76, 1993 (PubMed).

Maeda, Yamagata, Nishikawa et al.: "Requirement of c-kit for development of intestinal pacemaker system." in: Development (Cambridge, England), Vol. 116, Issue 2, pp. 369-75, 1993 (PubMed).

Ikuta, Weissman: "Evidence that hematopoietic stem cells express mouse c-kit but do not depend on steel factor for their generation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 4, pp. 1502-6, 1992 (PubMed).

Takagi, Nakahata, Kubo et al.: "Stimulation of mouse connective tissue-type mast cells by hemopoietic stem cell factor, a ligand for the c-kit receptor." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 148, Issue 11, pp. 3446-53, 1992 (PubMed).

Rico-Vargas, Weiskopf, Nishikawa et al.: "c-kit expression by B cell precursors in mouse bone marrow. Stimulation of B cell genesis by in vivo treatment with anti-c-kit antibody." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 152, Issue 6, pp. 2845-52, 1994 (PubMed).

Fleming, Alpern, Uchida et al.: "Steel factor influences the distribution and activity of murine hematopoietic stem cells in vivo." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 90, Issue 8, pp. 3760-4, 1993 (PubMed).

Hosts (332), (157), (127), (2), (1)
Reactivities (436), (323), (155), (105), (104), (91), (27), (5)
Applications (258), (180), (115), (100), (89), (62), (34), (31), (25), (20), (10), (10), (7), (3), (2), (2)
Conjugates (45), (36), (36), (21), (19), (10), (10), (10), (10), (10), (10), (10), (10), (7), (6), (6), (4), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (34), (31), (30), (14), (14), (14), (13), (13), (13), (12), (12), (11), (9), (8), (4), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
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