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Insulin-Degrading Enzyme (IDE) (Internal Region) antibody

Details for Product No. ABIN334425
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Antigen
Synonyms zgc:162603, IDE, 1300012G03Rik, 4833415K22Rik, AA675336, AI507533, INSDEGM, INSULYSIN
Epitope
Internal Region
(16), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(65), (36), (26), (25), (25), (24), (4)
Host
Goat
(42), (15), (14)
Clonality
Polyclonal
Conjugate
Un-conjugated
(3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
(48), (21), (20), (13), (11), (9), (2), (1), (1), (1)
Pubmed 1 reference available
Quantity 100 µg
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Catalog No. ABIN334425
374.27 $
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Immunogen Peptide with sequence C-QQYNFDRDNTE, from the internal region of the protein sequence according to NP_004960.1, NP_001159418.1.
Specificity This antibody is expected to recognize both reported isoforms (NP_004960.1 and NP_001159418.1).
Cross-Reactivity Dog (Canine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name Insulysin / Insulinase
Background Synonyms: insulinase, insulysin, insulin-degrading enzyme, FLJ35968, INSULYSIN, IDE
Gene ID 3416
NCBI Accession NP_004960.1, NP_001159418.1
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 110-120 kDa band observed in lysates of cell line K562 (calculated MW of 118 kDa according to NP_004960.1). In transfected HEK293 transiently expressing Insulysin a band of approx. 110 kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.1-0.3 µg/mL.
IHC: In paraffin embedded Human Lung shows cytoplasm staining of alveolar macrophages. Recommended concentration: 3-6 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-Insulin-Degrading Enzyme (IDE) (Internal Region) antibody ABIN334425 (0.1µg/ml) staining of K562 cell lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
General Zhao, Xiang, Haroutunian et al.: "Insulin degrading enzyme activity selectively decreases in the hippocampal formation of cases at high risk to develop Alzheimer's disease." in: Neurobiology of aging, Vol. 28, Issue 6, pp. 824-30, 2007 (PubMed).

Validation Images
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