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AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (pSer473)

Details for Product anti-AKT1 Antibody No. ABIN349614, Supplier: Log in to see
Antigen
  • Akt
  • akt
  • AKT
  • akt-1
  • Akt/PKB
  • AKT/PKB
  • AKT1
  • akt1
  • Akt1
  • ATAKT1
  • CG4006
  • CWS6
  • D-Akt
  • dAKT
  • dakt
  • dAkt
  • Dakt
  • DAkt
  • dAKT/dPKB
  • dAkt/PKB
  • dakt1
  • dAkt1
  • dAKT1
  • DAkt1
  • DAKT1
  • Dakt1
  • DAKT1/PKB
  • Dmel\\CG4006
  • Dpkb
  • DPKB
  • dPKB
  • DRAC-PK
  • DRAC-PK66
  • DRAC-PK85
  • F18A8.2
  • F18A8_2
  • K+ transporter 1
  • l(3)04226
  • l(3)89Bq
  • p-Akt
  • pAkt
  • pkb
  • PKB
  • PKB-ALPHA
  • PKB/Akt
  • PKB/AKT
  • PKB/dAKT
  • PKBalpha
  • POTASSIUM TRANSPORTER
  • PRKBA
  • Rac
  • RAC
  • RAC-ALPHA
  • RacPK
  • v-akt
  • v-akt1
  • xAct
Alternatives
anti-Human AKT antibody for Western Blotting
Epitope
pSer473
141
114
88
85
54
39
38
38
26
25
21
20
18
16
15
15
14
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13
11
11
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5
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4
4
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
1064
473
381
43
41
31
30
20
17
15
10
6
5
4
2
2
1
1
1
Host
Mouse
878
207
14
4
1
Clonality (Clone)
Monoclonal ()
Conjugate
This AKT antibody is un-conjugated
40
33
33
32
27
25
5
4
4
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
879
515
413
201
109
107
107
71
42
36
24
12
11
6
3
3
3
3
1
1
1
1
1
Supplier
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Immunogen Anti-AKT pS473 (MOUSE) Monoclonal Antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding S473 of human AKT1 protein.
Clone 17F6-B11
Isotype IgG1
Cross-Reactivity Mouse (Murine), Rat (Rattus), Monkey
Characteristics Concentration Definition: by UV absorbance at 280 nm
Alternative Name Akt (AKT1 Antibody Abstract)
Background Phospho AKT antibody is suitable for ELISA, immunohistochemistry, immunoprecipitation and western blotting. AKT phospho 473 is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308. AKT is a cytoplasmic protein also known as AKT1, Protein Kinase B (PKB) and rac (related to A and C kinases). AKT is a key regulator of many signal transduction pathways. AKT Exhibits tight control over cell proliferation and cell viability. Overexpression or inappropriate activation of AKT is noted in many types of cancer. AKT mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus. AKT has two main roles: (i) inhibition of apoptosis; (ii) promotion of proliferation. Anti-AKT pS473 (MOUSE) Monoclonal Antibody is ideal for investigators involved in Cell Signaling, Cancer, Neuroscience, Signal Transduction research.
Synonyms: phospho AKT, RAC-PK-alpha, Protein kinase B, PKB, C-AKT, RAC-alpha serine/threonine-protein kinase, Proto-oncogene c-Akt, AKT1, AKT 1, AKT-1
Gene ID 207, 62241011
UniProt P31749
Pathways PI3K-Akt Signaling, RTK Signaling, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway
Application Notes Expect a band approximately 56 kDa in size corresponding to phosphorylated AKT protein by western blotting in the appropriate cell lysate or extract. This phospho-specific monoclonal antibody reacts with human and mouse AKT pS473 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide. Specific conditions for reactivity should be optimized by the end user. For immunohistochemistry use formalin-fixed paraffin-embedded sections. No pre-treatment of sample is required.
Protocol This monoclonal antibody is suitable for ELISA,immunohistochemistry, immunoprecipitation and western blotting. Expect a band approximately 56 kDa in size corresponding to phosphorylated AKT protein by western blotting in the appropriate cell lysate or extract. This phospho-specific monoclonal antibody reacts with human and mouse AKT pS473 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide. Specific conditions for reactivity should be optimized by the end user. For immunohistochemistry use formalin-fixed paraffinembedded sections. No pre-treatment of sample is required. ELISA 1:20,000; WESTERN BLOT 1:500 - 1:3,000; IMMUNOHISTOCHEMISTRY 20 μg/ml; OTHER APPLICATIONS User Optimized
Restrictions For Research Use only
Format Liquid
Concentration 1.02 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
Immunohistochemistry (IHC) image for anti-AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (pSer473) (ABIN349614) Immunohistochemistry of Mouse anti-AKT pS473 antibody. Tissue: human prostate tissue....
Western Blotting (WB) image for anti-AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (pSer473) (ABIN349614) Immunohistochemistry using Anti-AKT pS473 monoclonal antibody shows detection of phos...
 image for anti-AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (pSer473) (ABIN349614) anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (pSer473) antibody (Image 3)
 image for anti-AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (pSer473) (ABIN349614) anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (pSer473) antibody (Image 4)
 image for anti-AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (pSer473) (ABIN349614) anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (pSer473) antibody (Image 5)
 image for anti-AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (pSer473) (ABIN349614) anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (pSer473) antibody (Image 6)
 image for anti-AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (pSer473) (ABIN349614) anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (pSer473) antibody (Image 7)
Product cited in: Ito, Nishibori, Ito, Takagi, Akimoto, Kudo, Asanuma, Sai, Miyamoto, Takenaka, Yan: "mTORC1 activation triggers the unfolded protein response in podocytes and leads to nephrotic syndrome." in: Laboratory investigation; a journal of technical methods and pathology, 2011

Background publications Lawlor, Alessi: "PKB/Akt: a key mediator of cell proliferation, survival and insulin responses?" in: Journal of cell science, Vol. 114, Issue Pt 16, pp. 2903-10, 2001

Alessi: "Discovery of PDK1, one of the missing links in insulin signal transduction. Colworth Medal Lecture." in: Biochemical Society transactions, Vol. 29, Issue Pt 2, pp. 1-14, 2001

Jones, Jakubowicz, Pitossi, Maurer, Hemmings: "Molecular cloning and identification of a serine/threonine protein kinase of the second-messenger subfamily." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 88, Issue 10, pp. 4171-5, 1991

Staal: "Molecular cloning of the akt oncogene and its human homologues AKT1 and AKT2: amplification of AKT1 in a primary human gastric adenocarcinoma." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 84, Issue 14, pp. 5034-7, 1987

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