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Proliferating Cell Nuclear Antigen (PCNA) (C-Term) antibody

Antigen

Proliferating Cell Nuclear Antigen (PCNA)
Synonyms MGC8367, PCNAR, Pcna/cyclin, pcna
Binding Site
Alternatives

C-Term
Clonality Polyclonal
Host
Alternatives

Rabbit
Reactivity
Conjugate
Alternatives Un-conjugated
Application
Alternatives Immunohistochemistry (IHC), Western Blotting (WB)
4 references available
Catalog no. ABIN350772
Quantity 500 µg
Price 454.67 $   Plus shipping costs $45.00
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Additional Information
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Alternative name Prolifeing cell nuclear antigen (PCNA)
UniProt P04961
Immunogen A synthetic peptide from the c-terminal region of rat Proliferating cell nuclear antigen (PCNA) conjugated to an immunogenic carrier protein was used as the immunogen. The antigen is homologous in human and rat.
Format Lyophilized
Isotype IgG
Description Function: This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Involvement in disease: Antibodies are present in sera from patients with systemic lupus erythematosus. Also known as: Cyclin, DNA polymerase delta auxiliary protein, Polymerase delta accessory protein, Proliferating Cell Nuclear Antigen.
Specificity Appears to be specific for PCNA.

Application Details
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Application Notes IHC, WB. A concentration of 10-50 µg/ml is recommended. The optimal concentration should be determined by the end user. Not yet tested in other applications.
Purity IgG
Storage Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Restrictions For Research Use only

Publications
Application Details Alternatives back to top

Almendral, Huebsch, Blundell et al.: "Cloning and sequence of the human nuclear protein cyclin: homology with DNA-binding proteins." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 84, Issue 6, pp. 1575-9, 1987 (PubMed).

Deloukas, Matthews, Ashurst et al.: "The DNA sequence and comparative analysis of human chromosome 20." in: Nature, Vol. 414, Issue 6866, pp. 865-71, 2002 (PubMed).

Sakurai, Kitano, Yamaguchi et al.: "Structural basis for recruitment of human flap endonuclease 1 to PCNA." in: The EMBO journal, Vol. 24, Issue 4, pp. 683-93, 2005 (PubMed).

Unk, Hajdú, Fátyol et al.: "Human SHPRH is a ubiquitin ligase for Mms2-Ubc13-dependent polyubiquitylation of proliferating cell nuclear antigen." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 48, pp. 18107-12, 2006 (PubMed).

Alternatives
Application Details Publications back to top

Alternatives for antigen "Proliferating Cell Nuclear Antigen (PCNA)", type "Antibodies"
Hosts Rabbit (161), Mouse (134), Goat (3), Rat (2)
Reactivities Human (260), Rat (Rattus) (178), Mouse (Murine) (151), Cow (Bovine) (119), Chicken (95), Dog (Canine) (95), Sheep (Ovine) (36), Pig (Porcine) (23), Guinea Pig (18), Insect (7), Hamster (6), Vertebrates (5), Rabbit (4), Yeast (3), Yeast (Saccharomyces Cerevisiae) (3), Algae (2), Fish (2), Mammalian (2), Microalgae (Isochrysis galbana) (2), Monkey (2), Xenopus laevis (2), Amphibian (1), Cat (Feline) (1), Fruit Fly (Drosophila melanogaster) (1), Yeast (Pichia pastoris) (1), Zebrafish (1)
Applications Western Blotting (WB) (162), Immunofluorescence (IF) (114), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (94), ELISA (72), Immunoprecipitation (IP) (49), Immunohistochemistry (IHC) (43), Flow Cytometry (FACS) (35), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (15), Immunocytochemistry (ICC) (13), Immunohistochemistry (Fixed) (IHC (fx)) (8), Immunohistochemistry (Frozen Sections) (IHC (fro)) (8), Immunoelectron Microscopy (IEM) (6), Cellular Assay (CA) (1), Dot Blot (Dot) (1), Immunoassay (IA) (1), Immunostaining (ISt) (1)
Conjugates FITC (17), Biotin (15), PE (9), Alexa Fluor 350 (6), Alexa Fluor 488 (6), Alexa Fluor 555 (6), Alexa Fluor 647 (6), Cy3 (6), Cy5 (6), Cy5.5 (6), Cy7 (6), Gold (6), HRP (6), PE,Cy3 (6), PE,Cy5 (6), PE,Cy5.5 (6), PE,Cy7 (6), APC (1), RPE (1)
Epitopes C-Term (67), N-Term (18), pTyr211 (18), Internal Region (6), AA 55-71 (4), AA 1-261 (3), Center (2), AA 111-112 (1), AA 111-122 (1), AA 75-125 (1), C-Term,ubLys164 (1), Internal Region,AA 111-122 (1), Nuclear (1), Tyr211 (1), pGlu1,pThr395 (1)