BAD antibody (pSer118, pSer155)

Catalog No. ABIN356355

The Mouse Monoclonal anti-BAD antibody (Clone 27AT937) (ABIN356355) specifically detects BAD in WB and EIA. The antibody is reactive with Human and Mouse samples.

Quick Overview for BAD antibody (pSer118, pSer155) (ABIN356355)

Target: BAD (BCL2-Associated Agonist of Cell Death (BAD))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This BAD antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Clone: 27AT937

Product Details anti-BAD Antibody

Binding Specificity: pSer118, pSer155

Specificity: This antibody is specific to mBad pSer155.

Characteristics: Synonyms: BAD, BBC6, BCL2L8, Bcl-2-like protein 8, Bcl2-L-8, Bcl2 antagonist of cell death,Bcl-2-binding component 6, Bcl-XL/Bcl-2-associated death promoter

Purification: Protein G Chromatography eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Immunogen: This monoclonal antibody is generated from mice immunized with a KLH conjugatedsynthetic peptide corresponding to sequences in Ser155 region of mouse Bad and residueSer155 is phosphorylated.

Isotype: IgG1

Application Details

Application Notes: ELISA: 1/1,000. Western blotting: 1/100-1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS containing 0.09 % (W/V) Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for BAD

Target: BAD (BCL2-Associated Agonist of Cell Death (BAD))

Background: Apoptosis is a critical aspect of cellular function and is implicated in a broad range of human diseases, from cancer to neurodegenerative and cardiovascular diseases. Intrafamilial Bcl-2 protein contacts are thought to regulate key aspects of apoptosis. The corruption of apoptotic instructions is associated with a large subset of human diseases, ranging from cancer and cardiovascular disease to neurodegenerative diseases, and name just a few. Understanding regulation of apoptosis is necessary to constructing a strategy for pharmaceutical intervention. The BH3 domain of Bcl-2 family members has been identified as key to the Bcl-2 regulatory function, although details are not fully elucidated.Synonyms: BAD, BBC6, BCL2L8, Bcl-2-binding component 6, Bcl-2-like protein 8, Bcl-XL/Bcl-2-associated death promoter, Bcl2 antagonist of cell death, Bcl2-L-8

Gene ID: 12015

UniProt: Q61337

Pathways: MAPK Signaling, PI3K-Akt Signaling, RTK Signaling, Apoptosis, Fc-epsilon Receptor Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Hepatitis C, CXCR4-mediated Signaling Events