Vimentin (VIM) antibody

Antigen: Vimentin (VIM)

Synonyms: FLJ36605, MGC102095, cb28, MGC139781, VIM, vim, Vim, vime

Clonality: Polyclonal

Host: Chicken

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Application: Western Blotting (WB), Immunofluorescence (IF)

Catalog no.: ABIN361371

Additional Information

Alternative name: Vimentin

Description: Vimentin is the major protein subunit of the 10nm or intermediate filaments (IFs) found in many kinds of mesenchymal and epithelial cells as well as developing neuronal and astrocytic precursor cells in the CNS. Vimentin is thought to be critically involved in lymphocyte adhesion and transmigration (Nieminen M et al. 2006). Copolymers are frequently formed between vimentin and other IFs, such as GFAP (in many kinds of astrocytes), desmin (in muscle cells) and neurofilament proteins (in developing neurons). Antibodies to vimentin are useful in studies of stem cells and generally to reveal the filamentous cytoskeleton. Recent studies suggest that vimentin affects prostate cancer cells motility and invasiveness (Zhao et al. 2008).

Specificity: Specific for the ~50kDa Vimentin protein. It is expected that it will work on other mammalian tissues.

Application Details

Application Notes: Recommended Dilution: WB: 1:1,000 IF: 1:500 Quality Control: Western blots performed on each lot.

Purification: Total IgY fraction.

Storage: Store at -20 °C, stable for 1 year

Research Area: Lineage Markers, Cytoskeleton, Neural Stem Cell marker, Cell/Tissue Markers

Restrictions: For Research Use only

Images

Western blot of NIH 3T3 cells showing specific immunolabeling of the ~50k Vimentin protein.

Mixed neuron/glial cultures stained with anti-Vimentin (green) and rabbit anti-GFAP antibody (620-GFAP) (red). Vimentin is expressed alone in fibroblastic and endothelial cells, which are the flattened cells in the middle of the image which appear green. Astrocytes may express primarily GFAP, or GFAP and vimentin, and so appear red (GFAP only) or golden yellow (GFAP and Vimentin). In cells which express both GFAP and vimentin, the two proteins assemble to produce heteropolymer filaments.