PLK1 antibody (pThr210)

Catalog No. ABIN361457

Product Details anti-PLK1 Antibody

Target: PLK1 (Polo-Like Kinase 1 (PLK1))

Binding Specificity: pThr210

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PLK1 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: Specific for ~66k PLK phosphorylated at Thr210. Immunolabeling of the PLK band is completely blocked by (-phosphatase treatment.

Cross-Reactivity: Human, Rat (Rattus)

Predicted Reactivity: bovine, canine, mouse, non-human primate, Xenopus, Zebrafish

Purification: Antigen Affinity Purified from Pooled Serum

Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr210 conjugated to KLH

Application Details

Application Notes: Recommended Dilution: WB: 1:1000 Quality Control: Western blots performed on each lot.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 μL in 10 mM HEPES (  pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.

Storage: -20 °C

Target Details for PLK1

Target: PLK1 (Polo-Like Kinase 1 (PLK1))

Alternative Name: PLK1 (PLK1 Products)

Synonyms: PLK antibody, STPK13 antibody, plk antibody, PLK-1 antibody, Plx1 antibody, stpk13 antibody, Plk antibody, cb525 antibody, cb636 antibody, ik:tdsubc_2d1 antibody, wu:fb37g11 antibody, wu:fb76g03 antibody, xx:tdsubc_2d1 antibody, polo like kinase 1 antibody, polo like kinase 1 S homeolog antibody, polo-like kinase 1 antibody, polo-like kinase 1 (Drosophila) antibody, Serine/threonine-protein kinase plk-1 antibody, PLK1 antibody, plk1.S antibody, plk1 antibody, Plk1 antibody, plk-1 antibody

Background: Polo-like kinases are important regulators of cell cycle progression. PLK1 is a highly conserved Ser/Thr kinase that has essential roles in the formation of mitotic bipolar spindles (van Vugt et al., 2004). Deregulated expression of PLK?s is detected in many types of cancer and associated with oncogenesis (Takei et al., 2005). It has been proposed that PLK1 function is altered at different stages of mitosis through consecutive phosphorylation events at Ser137 and Thr210 (van de Weerdt et al., 2005). Anti-Phospho-Thr210 PLK1 Western blot of rat synaptic membrane showing specific immunolabeling of the ~66 k PLK protein phosphorylated at Thr210 (control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: (-Ptase). The blot is identical to the control except that it was incubated in (-Ptase (1200 units for 30 min) before being exposed to the phospho-Thr210 PLK antibody. The immunolabeling is completely eliminated by treatment with (-Ptase.

Molecular Weight: '66 kDa

Gene ID: 5347

UniProt: P53350

Pathways: Cell Division Cycle, M Phase