STAT1 antibody (pTyr701)

Catalog No. ABIN361957

This Rabbit Polyclonal antibody specifically detects STAT1 in WB and IHC. It exhibits reactivity toward Human, Mouse and Rat. It has been mentioned in 3+ publications

Quick Overview for STAT1 antibody (pTyr701) (ABIN361957)

Target: STAT1 (Signal Transducer and Activator of Transcription 1, 91kDa (STAT1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This STAT1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-STAT1 Antibody

Binding Specificity: pTyr701

Specificity: The antibody detects endogenous level of STAT1 only when phosphorylated at tyrosine 701.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Immunogen: Peptide sequence around phosphorylation site of pTyr701 (T-G-Y (p) -I-K) derived from Human STAT1. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

Isotype: IgG

Application Details

Application Notes: Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

References for anti-STAT1 antibody (ABIN361957)

Hara, Nakamura, Ninomiya, Mochizuki, Kamiya, Aizenman, Koketsu, Adachi: "Inhibitory effects of chalcone glycosides isolated from Brassica rapa L. 'hidabeni' and their synthetic derivatives on LPS-induced NO production in microglia." in: Bioorganic & medicinal chemistry, Vol. 19, Issue 18, pp. 5559-68, (2011) (PubMed).

Fros, Liu, Prow, Geertsema, Ligtenberg, Vanlandingham, Schnettler, Vlak, Suhrbier, Khromykh, Pijlman: "Chikungunya virus nonstructural protein 2 inhibits type I/II interferon-stimulated JAK-STAT signaling." in: Journal of virology, Vol. 84, Issue 20, pp. 10877-87, (2010) (PubMed).

Chen, Ma, Sytwu, Wang, Chen, Liu, Chen, Chen, Wang: "Neural stem cells secrete factors that promote auditory cell proliferation via a leukemia inhibitory factor signaling pathway." in: Journal of neuroscience research, Vol. 88, Issue 15, pp. 3308-18, (2010) (PubMed).

Target Details for STAT1

Target: STAT1 (Signal Transducer and Activator of Transcription 1, 91kDa (STAT1))

Alternative Name: STAT1

Background: Signal transducer and activator of transcription that mediates signaling by interferons (IFNs). Following type I IFN (IFN-a and IFN-beta) binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state. In response to type II IFN (IFN-gamma), STAT1 is tyrosine- and serine-phosphorylated. It then forms a homodimer termed IFN-gamma-activated factor (GAF), migrates into the nucleus and binds to the IFN gamma activated sequence (GAS) to drive the expression of the target genes, inducing a cellular antiviral state.

Molecular Weight: 91 kDa

UniProt: P42224

Pathways: JAK-STAT Signaling, RTK Signaling, Interferon-gamma Pathway, Response to Growth Hormone Stimulus, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Endopeptidase Activity, Hepatitis C, CXCR4-mediated Signaling Events