»
Antibody
»
anti-BCL2-Associated Agonist of Cell Death BAD pSer112
BCL2-Associated Agonist of Cell Death (BAD) (pSer112) antibody
| Antigen |
|
| Synonyms |
BBC2, BCL2L8, wu:fa01b12, wu:fa96d04, MGC72439, BAD, MGC127164 |
|
Epitope
|
pSer112/75 (7), pSer155/118 (6), Ab-136 (5), Ab-155 (5), pSer136 (5), pSer155 (5), pSer112 (4), pSer136/99 (4), BH3 Domain (2), C-Term (2), Center (2), Non PSer134 (1), PSer170/134 (1), cleaved (1), pS118 (1), pS134 (1), pS75 (1), pS99 (1), pSer118 (1), pSer134 (1), pSer75 (1), pSer99 (1), prylated at serine 112 (1)
|
| Clonality |
Polyclonal |
|
Host
|
|
|
Reactivity
|
|
|
Application
|
 Alternatives Western Blotting (WB), Immunohistochemistry (IHC)
Western Blotting (WB) (120), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (55), Immunohistochemistry (IHC) (48), Immunofluorescence (IF) (31), Flow Cytometry (FACS) (28), Enzyme Immunoassay (EIA) (25), ELISA (23), Immunoprecipitation (IP) (19), Immunohistochemistry (Frozen Sections) (IHC (fro)) (8), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (4), ELISA (Detection) (2), Immunoelectron Microscopy (EM) (2), Dot Blot (Dot) (1), Immunocytochemistry (ICC) (1)
|
| Catalog no. |
ABIN361999 |
| Quantity |
50µg (50µg/50µl) (Variants) |
| Price |
280.00 $ Plus shipping costs $35.00
|
| Shipping to |
|
| Availability |
Ships within 5 Business days |
|
Alternative name
|
BAD (Phospho-Ser112)
|
|
Swiss-Prot
|
Q61337
|
|
Immunogen
|
The antiserum was produced against synthesized phosphopeptide derived from mouse BAD around the phosphorylation site of serine 112 (H-S-S P -Y-P).
|
|
Isotype
|
IgG (Matching secondary antibodies)
|
|
Specificity
|
BAD (Phospho-Ser112) (Human) antibody detects endogenous levels of BAD only when phosphorylated at serine 112.
|
|
Application Notes
|
WB: 1:500~1:1000 IHC: 1:50~1:100
|
|
Concentration
|
50µg/50µl
|
|
Purification
|
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
|
|
Buffer
|
Rabbit IgG in phosphate buffered saline (without Mg 2+ and Ca 2+ ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
|
|
Storage
|
Store at -20°C. Avoid repeat freeze-thaw cycles.
|
|
Restrictions
|
For Research Use only
|
Alternatives for antigen "BCL2-Associated Agonist of Cell Death (BAD)", type "Antibodies"
|
Hosts
|
Rabbit (144), Mouse (12), Chicken (1)
|
|
Reactivities
|
Human (146), Mouse (Murine) (102), Rat (Rattus) (91), Monkey (1)
|
|
Applications
|
Western Blotting (WB) (120), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (55), Immunohistochemistry (IHC) (48), Immunofluorescence (IF) (31), Flow Cytometry (FACS) (28), Enzyme Immunoassay (EIA) (25), ELISA (23), Immunoprecipitation (IP) (19), Immunohistochemistry (Frozen Sections) (IHC (fro)) (8), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (4), ELISA (Detection) (2), Immunoelectron Microscopy (EM) (2), Dot Blot (Dot) (1), Immunocytochemistry (ICC) (1)
|
|
Conjugates
|
Alexa Flour 488 (2), Biotin (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), FITC (2), Gold (2), HRP (2), PE (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy7 (2), RBITC (2)
|
|
Epitopes
|
pSer112/75 (7), pSer155/118 (6), Ab-136 (5), Ab-155 (5), pSer136 (5), pSer155 (5), pSer112 (4), pSer136/99 (4), BH3 Domain (2), C-Term (2), Center (2), Non PSer134 (1), PSer170/134 (1), cleaved (1), pS118 (1), pS134 (1), pS75 (1), pS99 (1), pSer118 (1), pSer134 (1), pSer75 (1), pSer99 (1), prylated at serine 112 (1)
|
