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MEK1 antibody (pSer221)
MAP2K1
Reactivity: Human, Mouse, Rat
WB, IHC, IF
Host: Rabbit
Polyclonal
unconjugated
Product Details anti-MEK1 Antibody
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Target
See all MEK1 (MAP2K1) Antibodies
MEK1 (MAP2K1)
(Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1))
Binding Specificity
All epitopes for MEK1 antibodies
pSer221
Reactivity
All reactivities for MEK1 antibodies
Human, Mouse, Rat
Host
All hosts for MEK1 antibodies
Rabbit
Clonality
All clonalities for MEK1 antibodies
Polyclonal
Conjugate
All conjugates for MEK1 antibodies
This MEK1 antibody is un-conjugated
Application
All applications for MEK1 antibodies
Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Specificity
The antibody detects endogenous level of MEK1 only when phosphorylated at serine 221.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Immunogen
Peptide sequence around phosphorylation site of pSer221 (A-N-S (p) -F-V) derived from Human MEK1. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
Isotype
IgG
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Alternatives
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Application Details
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Application Notes
Western blotting: 1:500-1:1000 Immunohistochemistry: 1:50-1:100 Immunofluorescence: 1:100-1:200
Restrictions
For Research Use only
Handling
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Format
Liquid
Concentration
1 mg/mL
Buffer
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C/-20 °C
Storage Comment
Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
References for anti-MEK1 antibody (ABIN362167)
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Ikari, Atomi, Kinjo, Sasaki, Sugatani : "Magnesium deprivation inhibits a MEK-ERK cascade and cell proliferation in renal epithelial Madin-Darby canine kidney cells. " in: Life sciences , Vol. 86 , Issue 19-20 , pp. 766-73 , (2010 ) (PubMed ).
Target Details for MEK1
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Target
MEK1 (MAP2K1)
(Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1))
Alternative Name
MEK1 (MAP2K1 Products )
Synonyms
Mek1 antibody, CFC3 antibody, MAPKK1 antibody, MEK1 antibody, MKK1 antibody, PRKMK1 antibody, MEKK1 antibody, Prkmk1 antibody, mek-2 antibody, si:ch211-242m18.2 antibody, wu:fj56a12 antibody, wu:fj61b01 antibody, zgc:56557 antibody, ATMEK1 antibody, F20B18.180 antibody, F20B18_180 antibody, MAP kinase/ ERK kinase 1 antibody, MITOGEN ACTIVATED PROTEIN KINASE KINASE antibody, MITOGEN ACTIVATED PROTEIN KINASE KINASE 1 antibody, NMAPKK antibody, MEK antibody, mitogen activated protein kinase kinase 1 antibody, mitogen-activated protein kinase kinase 1 antibody, mitogen-activated protein kinase kinase 1 L homeolog antibody, MAP kinase/ ERK kinase 1 antibody, Map2k1 antibody, MAP2K1 antibody, map2k1.L antibody, map2k1 antibody, MEK1 antibody
Background
Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates ERK1 and ERK2 MAP kinases.
Molecular Weight
45 kDa
NCBI Accession
NP_002746
UniProt
Q02750
Pathways
MAPK Signaling , RTK Signaling , Interferon-gamma Pathway , Fc-epsilon Receptor Signaling Pathway , Neurotrophin Signaling Pathway , Activation of Innate immune Response , Toll-Like Receptors Cascades , Autophagy , Signaling of Hepatocyte Growth Factor Receptor , BCR Signaling
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