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Insulin Receptor Substrate 1 (IRS1) (pSer636) antibody

Details for Product No. ABIN362277, Supplier: Log in to see
Antigen
  • irs1
  • irsu
  • IRS1
  • HIRS-1
  • G972R
  • IRS-1
  • IRS1IRM
  • irs-1
  • irs1-a
  • insulin receptor substrate 2
  • insulin receptor substrate 1
  • irs2
  • IRS1
  • Irs1
  • irs1
Alternatives
anti-Human Insulin Receptor Substrate 1 antibody for Enzyme Immunoassay
Epitope
pSer636
57
50
45
45
26
23
21
21
17
15
15
14
13
13
13
13
13
12
9
9
8
7
6
5
5
4
4
4
4
4
3
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
543
381
359
13
8
5
1
1
1
Host
Rabbit
542
32
Clonality
Polyclonal
Conjugate
Un-conjugated
13
11
11
11
10
10
10
10
10
10
10
10
2
1
1
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
332
242
152
116
110
108
43
29
12
4
3
2
1
1
Options
Supplier
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Immunogen Peptide sequence around phosphorylation site of pSer636 (P-M-S (p) -P-K) derived from Human IRS-1. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
Isotype IgG
Specificity The antibody detects endogenous level of total IRS-1 protein.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Alternative Name IRS-1 (IRS1 Antibody Abstract)
Background May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit
Molecular Weight 180 kDa
NCBI Accession NP_005535
UniProt P35568
Pathways Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
Application Notes Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
Background publications Tzatsos, Kandror: "Nutrients suppress phosphatidylinositol 3-kinase/Akt signaling via raptor-dependent mTOR-mediated insulin receptor substrate 1 phosphorylation." in: Molecular and cellular biology, Vol. 26, Issue 1, pp. 63-76, 2005 (PubMed).

Ozes, Akca, Mayo, Gustin, Maehama, Dixon, Donner: "A phosphatidylinositol 3-kinase/Akt/mTOR pathway mediates and PTEN antagonizes tumor necrosis factor inhibition of insulin signaling through insulin receptor substrate-1." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, Issue 8, pp. 4640-5, 2001 (PubMed).

Kadowaki: "Insights into insulin resistance and type 2 diabetes from knockout mouse models." in: The Journal of clinical investigation, Vol. 106, Issue 4, pp. 459-65, 2000 (PubMed).

Szanto, Kahn: "Selective interaction between leptin and insulin signaling pathways in a hepatic cell line." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, Issue 5, pp. 2355-60, 2000 (PubMed).