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Phospholipase C gamma 1 antibody (pTyr771)
PLCG1
Reactivity: Human, Mouse, Rat
IF
Host: Rabbit
Polyclonal
unconjugated
Product Details anti-Phospholipase C gamma 1 Antibody
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Target
See all Phospholipase C gamma 1 (PLCG1) Antibodies
Phospholipase C gamma 1 (PLCG1)
(phospholipase C, gamma 1 (PLCG1))
Binding Specificity
All epitopes for Phospholipase C gamma 1 antibodies
pTyr771
Reactivity
All reactivities for Phospholipase C gamma 1 antibodies
Human, Mouse, Rat
Host
All hosts for Phospholipase C gamma 1 antibodies
Rabbit
Clonality
All clonalities for Phospholipase C gamma 1 antibodies
Polyclonal
Conjugate
All conjugates for Phospholipase C gamma 1 antibodies
This Phospholipase C gamma 1 antibody is un-conjugated
Application
All applications for Phospholipase C gamma 1 antibodies
Immunofluorescence (IF)
Specificity
The antibody detects endogenous level of PLC-gamma1 only when phosphorylated at tyrosine 771.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Immunogen
Peptide sequence around phosphorylation site of pTyr771 (P-D-Y (p) -G-A) derived from Human PLC-g1. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
Isotype
IgG
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Alternatives
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Application Details
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Application Notes
Western blotting: 1:500-1:1000 Immunofluorescence: 1:100-1:200
Restrictions
For Research Use only
Handling
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Format
Liquid
Concentration
1 mg/mL
Buffer
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C/-20 °C
Storage Comment
Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
Target Details for Phospholipase C gamma 1
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Target
Phospholipase C gamma 1 (PLCG1)
(phospholipase C, gamma 1 (PLCG1))
Alternative Name
PLC-gamma1 (PLCG1 Products )
Synonyms
NCKAP3 antibody, PLC-II antibody, PLC1 antibody, PLC148 antibody, PLCgamma1 antibody, plc1 antibody, nckap3 antibody, plc-ii antibody, plc148 antibody, plcgamma1 antibody, PPLCA antibody, AI894140 antibody, Cded antibody, Plc-1 antibody, Plc-gamma1 antibody, Plcg-1 antibody, fj46f04 antibody, wu:fj46f04 antibody, wu:fj57a05 antibody, phospholipase C gamma 1 antibody, phospholipase C gamma 1 L homeolog antibody, 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1 antibody, phospholipase C, gamma 1 antibody, PLCG1 antibody, plcg1.L antibody, LOC100633163 antibody, Plcg1 antibody, plcg1 antibody
Background
PLC-gamma is a major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase.
Molecular Weight
155 kDa
NCBI Accession
NP_002651
UniProt
P19174
Pathways
RTK Signaling , WNT Signaling , TCR Signaling , Fc-epsilon Receptor Signaling Pathway , EGFR Signaling Pathway , Neurotrophin Signaling Pathway , Thyroid Hormone Synthesis , Inositol Metabolic Process , Myometrial Relaxation and Contraction , Regulation of Muscle Cell Differentiation , Regulation of G-Protein Coupled Receptor Protein Signaling , Skeletal Muscle Fiber Development , G-protein mediated Events , Signaling Events mediated by VEGFR1 and VEGFR2 , Interaction of EGFR with phospholipase C-gamma , VEGFR1 Specific Signals , VEGF Signaling
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